Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type

• Published in: Stem cell reports 2020-07, Vol.15 (1), p.171-184
• Main Authors: Lee, Jeoung Eun, Lee, Ji Yoon, Park, Chang-Hwan, Eum, Jin Hee, Jung, Soo Kyung, Han, A-Reum, Seol, Dong-Won, Lee, Jin Saem, Shin, Hyun Soo, Im, Jung Ho, Chun, Taehoon, Ha, Kyungsoo, Heo, Deok Rim, Yoon, Tae Ki, Lee, Dong Ryul
• Format: Article
• Language: English
• Subjects: frozen cord blood cells; frozen oocytes; homozygous HLA; pluripotent stem cells; somatic cell nuclear transfer
• ISSN: 2213-6711; 2213-6711
• DOI: 10.1016/j.stemcr.2020.05.005

Human pluripotent stem cells (PSCs) through somatic cell nuclear transfer (SCNT) may be an important source for regenerative medicine. The low derivation efficiency of stem cells and the accessibility of human oocytes are the main obstacles to their application. We previously reported that the efficiency of SCNT was increased by overexpression of H3K9me3 demethylase. Here, we applied a modified derivation method to the PSC line and first obtained human SCNT-PSC lines derived from both donated cryopreserved oocytes and cord blood cells with a homozygous human leukocyte antigen (HLA) type. The SCNT-PSCs have very similar characteristics with embryonic stem cells (ESCs) and additionally have shown immunocompatibility in an in vitro and in vivo humanized mouse with a matching HLA type. Our study demonstrates that SCNT technology using donated cryopreserved oocytes and cord blood cells with a known HLA type provides a promising method for establishing a human HLA-matched SCNT-PSC bank for regenerative medicine. •Human normal SCNT-PSC line with homozygous HLA type is derived from both donated cryopreserved oocytes and cord blood cells•SCNT-PSC derivation is improved by modified method using ESC-conditioned medium•Differentiated functional cells from SCNT-PSC with homozygous HLA type have shown immunocompatibility in humanized mouse with a matching HLA type•This SCNT technology facilitates the establishment of the human HLA-matched SCNT-PSC bank In this article, Lee and his colleagues reported the successful production of HLA-homozygous SCNT-PSCs using MNCs from frozen/thawed donated cord blood in a public bank and frozen (vitrified) human oocytes donated after storage for 5 years. Homozygous SCNT-PSC-derived cells are highly immunocompatible with allogeneic HLA-matched immune cells. Therefore, we may suggest that this SCNT technology facilitates the establishment of the human HLA-matched SCNT-PSC bank.