MiR-221/222 promote migration and invasion, and inhibit autophagy and apoptosis by modulating ATG10 in aggressive papillary thyroid carcinoma

MicroRNA (miRNA) has been reported to exert important functions in papillary thyroid carcinomas (PTC). However, the role of miRNA in aggressive PTC (APTC) remains unclear. Here, we investigated the diagnostic potentials and mechanisms of miR-221/222 in APTC. Results showed that miR-221/222 were mark...

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Bibliographic Details
Published in:3 Biotech 2020-08, Vol.10 (8), p.339-339, Article 339
Main Authors: Shen, Hao, Lin, Zaikai, Shi, Haiyan, Wu, Lingling, Ma, Baojin, Li, Hong, Yin, Baobing, Tang, Jun, Yu, Hongjin, Yin, Xiaoxing
Format: Article
Language:English
Subjects:
Agriculture
Apoptosis
Autophagy
Bioinformatics
Biomaterials
Biotechnology
Cancer Research
Chemistry
Chemistry and Materials Science
Diagnostic systems
Fluorescence
Lymph nodes
Lymphatic system
Metastases
Metastasis
MicroRNAs
miRNA
mRNA
Original
Original Article
Papillary thyroid carcinoma
Phagocytosis
Proteins
Stem Cells
Thyroid
Thyroid cancer
Thyroid gland
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Summary:MicroRNA (miRNA) has been reported to exert important functions in papillary thyroid carcinomas (PTC). However, the role of miRNA in aggressive PTC (APTC) remains unclear. Here, we investigated the diagnostic potentials and mechanisms of miR-221/222 in APTC. Results showed that miR-221/222 were markedly up-regulated in PTC, compared with the adjacent normal tissue (ANT). A high expression of miR-221/222 were associated with a primary tumor, regional lymph node, and distant metastasis (TNM) stage, multicentricity, lymph node metastasis, and extra-thyroidal extension. Receiver operating characteristic (ROC) curve analysis indicated that miR-221/222 could be used as APTC diagnostic markers. Moreover, miR-221/222 tremendously promoted migration and invasion and inhibited apoptosis and autophagy in PTC cells. A luciferase assay showed that miR-221/222 inhibited the fluorescent activity of autophagy-related protein 10 (ATG10). Furthermore, miR-221/222 decreased ATG10 mRNA and protein levels. Silencing of ATG10 significantly abrogated the effect of miR-221/222 on apoptosis and autophagy. We suggested that miR-221/222 can promote migration and invasion, and inhibit autophagy and apoptosis by targeting ATG10 in APTC.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-020-02326-x