The amino-terminal region of dense granule protein 6 of Toxoplasma gondii stimulates IFN-γ production by microglia

We examined activities of dense granule proteins (GRAs), which Toxoplasma gondii secretes within infected cells, to stimulate microglial IFN-γ production in vitro. We identified that the N-terminal region (amino acids 41–152) of GRA6 (GRA6Nt) stimulates IFN-γ production by both a microglia cell line...

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Bibliographic Details
Published in:Microbes and infection 2020-09, Vol.22 (8), p.375-378
Main Authors: Sa, Qila, Mercier, Corinne, Cesbron-Delauw, Marie-France, Suzuki, Yasuhiro
Format: Article
Language:English
Subjects:
Biodiversity
Dense granule protein 6
IFN-gamma
Innate immunity
Life Sciences
Microglia
Populations and Evolution
Protective immunity
Toxoplasma
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Summary:We examined activities of dense granule proteins (GRAs), which Toxoplasma gondii secretes within infected cells, to stimulate microglial IFN-γ production in vitro. We identified that the N-terminal region (amino acids 41–152) of GRA6 (GRA6Nt) stimulates IFN-γ production by both a microglia cell line and primary microglia purified from the brains of uninfected adult mice. In contrast, neither of GRA1, GRA2, GRA5Nt, nor the carboxyl-terminal (amino acids 174–224) of GRA6 stimulated microglial IFN-γ production. GRA6Nt appears to be a target molecule of the sentinel function of microglia to detect cerebral proliferation of T. gondii and activate their IFN-γ production for facilitating the protective immunity to control the pathogen.
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2019.12.003