Increment of Lysosomal Biogenesis by Combined Extracts of Gum Arabic, Parsley, and Corn Silk: A Reparative Mechanism in Mice Renal Cells

Gum Arabic (GA), parsley, and corn silk have been traditionally used for renal failure patients worldwide. This study aimed at probing the mechanism of the combined extracts, namely, GA (3 g/kg/day), parsley (1 g/kg/day), and corn silk (200 mg/kg/day), as nephroprotective agents in mice after amikac...

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Published in:Evidence-based complementary and alternative medicine 2020, Vol.2020 (2020), p.1-12
Main Authors: Singab, Abdel Nasser B., Elshamy, Ali, Salem, Mohammad Alaraby, Tash, Reham, Abdelmohsen, Usama Ramadan, Rabeh, Mohamed, Omar, Nesreen, El-Shazly, Mohamed, Helmy, Aya, Samir, Ahmed
Format: Article
Language:English
Subjects:
Amikacin
Antibacterial agents
Antibiotics
Autophagy
BAX protein
Bcl-2 protein
Bcl-x protein
Biosynthesis
Cathepsin D
Cell death
Corn
Creatinine
Drinking water
Drug dosages
Gums
Herbs
Kidneys
Laboratory animals
LAMP-1 protein
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Membrane proteins
Pharmacy
Proteins
Renal failure
Silk
Western blotting
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Summary:Gum Arabic (GA), parsley, and corn silk have been traditionally used for renal failure patients worldwide. This study aimed at probing the mechanism of the combined extracts, namely, GA (3 g/kg/day), parsley (1 g/kg/day), and corn silk (200 mg/kg/day), as nephroprotective agents in mice after amikacin (1.2 g/kg) single dose through exploration of their action on G-protein coupled receptors (GPR) 41 and 43 and the ensuing lysosomal biogenesis. Western blotting was employed for renal levels of bcl-2-associated X protein (BAX) and cytosolic cathepsin D; cell death markers, nuclear transcription factor EB (TFEB), and lysosomal associated membrane protein-1 (LAMP-1); and lysosomal biogenesis indicators. Liquid chromatography–mass spectrometry (LC-MS) and docking were also employed. After amikacin treatment, BAX and cathepsin D levels were upregulated while LAMP-1 and nuclear TFEB levels were inhibited. The combined extracts inhibited BAX and cytosolic cathepsin D but upregulated LAMP-1 and nuclear TFEB levels. Docking confirmed GPR modulatory signaling. The combined extracts showed GPR signal modulatory properties that triggered lysosome synthesis and contributed to reversing the adverse effects of amikacin on renal tissues.
ISSN:1741-427X
1741-4288
DOI:10.1155/2020/8631258