Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury

Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal i...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2020-07, Vol.21 (13), p.4774
Main Authors: Lee, Jun Ho, Ha, Dae Hyun, Go, Hyeon-Kyu, Youn, Jinkwon, Kim, Hyun-Keun, Jin, Richard C, Miller, Randy B, Kim, Do-Hyung, Cho, Byong Seung, Yi, Yong Weon
Format: Article
Language:English
Subjects:
Acute Kidney Injury - chemically induced
Acute Kidney Injury - therapy
Adipose tissue
Adipose Tissue - cytology
Animal models
Animals
Biological Therapy - methods
Body fat
Cells, Cultured
Cisplatin
Exosomes
Good Manufacturing Practice
Humans
Injuries
Kidney diseases
Kidneys
Lipids
Mass production
Mesenchymal Stem Cells
Mesenchyme
Morphology
Productivity
Proteins
Rats
Renal failure
Stromal cells
Therapeutic applications
Ultracentrifugation
Umbilical cord
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal injuries in multiple experimental animal models of kidney diseases including AKI. However, the mass production of exosomes is a challenge not only in preclinical studies with large animals but also for successful clinical applications. In this respect, tangential flow filtration (TFF) is suitable for good manufacturing practice (GMP)-compliant large-scale production of high-quality exosomes. Until now, no studies have been reported on the use of TFF, but rather ultracentrifugation has been almost exclusively used, to isolate exosomes for AKI therapeutic application in preclinical studies. Here, we demonstrated the reproducible large-scale production of exosomes derived from adipose tissue-derived MSC (ASC-exosomes) using TFF and the lifesaving effect of the ASC-exosomes in a lethal model of cisplatin-induced rat AKI. Our results suggest the possibility of large-scale stable production of ASC-exosomes without loss of function and their successful application in life-threatening diseases.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21134774