Molecular detection and phylogenetic analyses of Wolbachia in natural populations of nine galling Aphid species

Wolbachia is one of the most abundant facultative intracellular symbionts in arthropods. It alters host biology in diverse ways, including the induction of reproductive manipulation, association of nutrient supplier and protection against pathogens. Aphids are a group of insects which exhibit intere...

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Bibliographic Details
Published in:Scientific reports 2020-07, Vol.10 (1), p.12025-12025, Article 12025
Main Authors: Ren, Weibin, Wei, Hongyuan, Yang, Ying, Shao, Shuxia, Wu, Haixia, Chen, Xiaoming, Yang, Zixiang
Format: Article
Language:English
Subjects:
631/326/41
631/326/421
Animals
Aphidoidea
Aphids - microbiology
Asexual reproduction
Developmental stages
Galls
Genes, Bacterial
Genome, Bacterial
Host plants
Humanities and Social Sciences
Insects
Life cycles
Microenvironments
multidisciplinary
Natural populations
Phylogeny
Population studies
RNA, Bacterial
RNA, Ribosomal, 16S - genetics
Science
Science (multidisciplinary)
Species
Symbionts
Wolbachia
Wolbachia - classification
Wolbachia - genetics
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Summary:Wolbachia is one of the most abundant facultative intracellular symbionts in arthropods. It alters host biology in diverse ways, including the induction of reproductive manipulation, association of nutrient supplier and protection against pathogens. Aphids are a group of insects which exhibit interesting biological characteristics such as complex life cycles, alteration of sexual and asexual reproduction and shifts between two different hosts. Wolbachia is widely present in many orders of insects, but so far limited studies on Wolbachia in aphids have been carried out. Galling aphids are a group of aphids that induce galls on their primary host plants at specific life stage. In this study, 15 natural populations representing nine galling aphid species were analyzed for the presence of Wolbachia using species-specific primer pairs. Wolbachia presence in galling aphids was quite low and varied significantly among aphid populations. Only three of the 15 populations we analyzed had detectable Wolbachia and the overall infection rate was 20%. Two Wolbachia strains, O and B, were identified from the galling aphids Kaburagia rhusicola and Schlechtendalia chinensis . Strain O was for the first time to be found in aphids, and it is likely involved with the life stages of galling aphids living in closed microenvironments with specific survival strategies that are different from free-living aphids.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-68925-z