In situ hybridization of feline leukemia virus in a primary neural B-cell lymphoma

An 8-y-old castrated male, outdoor European shorthair cat was presented with a history of hindlimb weakness and paralysis. Disease progression was continuous from the onset; deep algesia disappeared at the final stage. Radiography of the vertebral column was unremarkable; along with patient history...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2020-05, Vol.32 (3), p.454-457
Main Authors: Szilasi, Anna, Dénes, Lilla, Jakab, Csaba, Erdélyi, Ildikó, Resende, Talita, Vannucci, Fabio, Csomor, Judit, Mándoki, Míra, Balka, Gyula
Format: Article
Language:English
Subjects:
Animals
Brief Communications
Cat Diseases - pathology
Cat Diseases - virology
Cats
In Situ Hybridization - veterinary
Leukemia Virus, Feline - genetics
Leukemia Virus, Feline - physiology
Lymphoma, B-Cell - pathology
Lymphoma, B-Cell - veterinary
Lymphoma, B-Cell - virology
Male
Retroviridae Infections - pathology
Retroviridae Infections - veterinary
Retroviridae Infections - virology
RNA, Viral - analysis
RNA, Viral - isolation & purification
Tumor Virus Infections - pathology
Tumor Virus Infections - veterinary
Tumor Virus Infections - virology
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Summary:An 8-y-old castrated male, outdoor European shorthair cat was presented with a history of hindlimb weakness and paralysis. Disease progression was continuous from the onset; deep algesia disappeared at the final stage. Radiography of the vertebral column was unremarkable; along with patient history and physical examination results, magnetic resonance imaging suggested inflammatory lesions in the spinal cord, although neoplasia could not be ruled out. Feline leukemia virus (FeLV) positivity was confirmed by a serum ELISA prior to euthanasia. Upon postmortem examination, hemorrhages were present in the spinal cord at the level of vertebrae T7-8. Histologic and immunohistochemical analysis revealed primary diffuse large B-cell lymphoma of the spinal cord with multifocal myelomalacia and hemorrhages. To determine the presence of a pathogen within the lesion, we developed a novel in situ hybridization protocol for FeLV (RNAscope). The reaction revealed large amounts of FeLV viral RNA in the tumor cells.
ISSN:1040-6387
1943-4936
DOI:10.1177/1040638720915449