Trajectory analysis quantifies transcriptional plasticity during macrophage polarization

In recent years, macrophages have been shown to be tremendously plastic in both in vitro and in vivo settings; however, it remains unclear whether macrophages retain any persistent memory of past polarization states which may then impact their future repolarization to new states. Here, we perform de...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2020-07, Vol.10 (1), p.12273-12273, Article 12273
Main Authors: Liu, Serena X., Gustafson, Heather H., Jackson, Dana L., Pun, Suzie H., Trapnell, Cole
Format: Article
Language:English
Subjects:
631/337
631/337/2019
631/80
Animals
Biomarkers
Bone marrow
Cell growth
Chromatin Assembly and Disassembly
Cytokines
Cytokines - genetics
Disease
Gene Expression Profiling - methods
Genes
Genomes
Genotype & phenotype
Humanities and Social Sciences
Humans
Macrophage Activation - genetics
Macrophage Activation - immunology
Macrophages
Macrophages - immunology
Macrophages - metabolism
Medical research
Mice
multidisciplinary
Phenotype
Phenotypes
Plasticity
Polarization
Science
Science (multidisciplinary)
Transcription
Transcriptome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In recent years, macrophages have been shown to be tremendously plastic in both in vitro and in vivo settings; however, it remains unclear whether macrophages retain any persistent memory of past polarization states which may then impact their future repolarization to new states. Here, we perform deep transcriptomic profiling at high temporal resolution as macrophages are polarized with cytokines that drive them into “M1” and “M2” molecular states. We find through trajectory analysis of their global transcriptomic profiles that macrophages which are first polarized to M1 or M2 and then subsequently repolarized demonstrate little to no memory of their polarization history. We observe complete repolarization both from M1 to M2 and vice versa, and we find that macrophage transcriptional phenotypes are defined by the current cell microenvironment, rather than an amalgamation of past and present states.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-68766-w