Overexpression of p21 Has Inhibitory Effect on Human Hematopoiesis by Blocking Generation of CD43＋ Cells via Cell-Cycle Regulation

p21, an important member of the Cip/Kip family, is involved in inhibitory effects of overexpression during the early stage of human hematopoiesis. We established a human embryonic stem cell (hESC) line with inducible expression of p21 (p21/hESCs). Overexpression of p21 did not influence either mesod...

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Published in:International journal of stem cells 2020-07, Vol.13 (2), p.202-211
Main Authors: Zeng, Jiahui, Zhang, Huifang, Liu, Yuanling, Sun, Wencui, Yi, Danying, Zhu, Lijiao, Zhang, Yonggang, Pan, Xu, Chen, Yijing, Zhou, Ya, Bian, Guohui, Lai, Mowen, Zhou, Qiongxiu, Liu, Jiaxin, Chen, Bo, Ma, Feng
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Language:English
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Summary:p21, an important member of the Cip/Kip family, is involved in inhibitory effects of overexpression during the early stage of human hematopoiesis. We established a human embryonic stem cell (hESC) line with inducible expression of p21 (p21/hESCs). Overexpression of p21 did not influence either mesoderm induction or emergence of CD34＋ cells, but it significantly decreased the production of CD43＋ cells and changed the expression profile of hematopoiesis-related factors, leading to the negative effects of p21 on hematopoiesis. In /hESC co-cultures when was induced from D0, perturbation of the cell cycle caused by upregulation of p21 probably prevented the appearance of CD43＋ cells, but not CD34＋ cells. The mechanisms via which CD34＋ cells are blocked by overexpression remain to be elucidated.
ISSN:2005-3606
2005-5447
DOI:10.15283/ijsc20033