High-Performance Molecular Imaging with MALDI Trapped Ion-Mobility Time-of-Flight (timsTOF) Mass Spectrometry

Imaging mass spectrometry (IMS) enables the spatially targeted molecular assessment of biological tissues at cellular resolutions. New developments and technologies are essential for uncovering the molecular drivers of native physiological function and disease. Instrumentation must maximize spatial...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-11, Vol.91 (22), p.14552-14560
Main Authors: Spraggins, Jeffrey M, Djambazova, Katerina V, Rivera, Emilio S, Migas, Lukasz G, Neumann, Elizabeth K, Fuetterer, Arne, Suetering, Juergen, Goedecke, Niels, Ly, Alice, Van de Plas, Raf, Caprioli, Richard M
Format: Article
Language:English
Subjects:
Adducts
Chemistry
Data acquisition
Instrumentation
Ionic mobility
Ionization
Ions
Kidneys
Lipids
Mass spectrometry
Mass spectroscopy
Medical imaging
Mobility
Neuroimaging
Pixels
Resolution
Scientific imaging
Spatial data
Spatial discrimination
Spatial resolution
Spectroscopy
Tissues
Vapor phases
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Summary:Imaging mass spectrometry (IMS) enables the spatially targeted molecular assessment of biological tissues at cellular resolutions. New developments and technologies are essential for uncovering the molecular drivers of native physiological function and disease. Instrumentation must maximize spatial resolution, throughput, sensitivity, and specificity, because tissue imaging experiments consist of thousands to millions of pixels. Here, we report the development and application of a matrix-assisted laser desorption/ionization (MALDI) trapped ion-mobility spectrometry (TIMS) imaging platform. This prototype MALDI timsTOF instrument is capable of 10 μm spatial resolutions and 20 pixels/s throughput molecular imaging. The MALDI source utilizes a Bruker SmartBeam 3-D laser system that can generate a square burn pattern of
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b03612