Lymphocyte activation gene-3 (LAG3) mRNA and protein expression on tumour infiltrating lymphocytes (TILs) in oesophageal adenocarcinoma

Purpose Lymphocyte activation gene-3 (LAG3) is an immunosuppressive checkpoint molecule expressed on T cells. The frequency and distribution of LAG3 expression in oesophageal adenocarcinoma (EAC) is unknown. Aim of the study was the evaluation and distribution of LAG3 on tumour infiltrating lymphocy...

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Bibliographic Details
Published in:Journal of cancer research and clinical oncology 2020-09, Vol.146 (9), p.2319-2327
Main Authors: Gebauer, Florian, Krämer, Max, Bruns, Christiane, Schlößer, Hans A., Thelen, Martin, Lohneis, Philipp, Schröder, Wolfgang, Zander, Thomas, Alakus, Hakan, Buettner, Reinhard, Loeser, Heike, Quaas, Alexander
Format: Article
Language:English
Subjects:
Adenocarcinoma
Cancer Research
CD223 antigen
CD4 antigen
CD8 antigen
Cell activation
DNA microarrays
Esophageal cancer
Esophagus
Gene expression
Hematology
Immunofluorescence
Immunohistochemistry
Immunomodulation
Internal Medicine
Lymphocytes
Lymphocytes T
Medicine
Medicine & Public Health
Oncology
Original Article – Cancer Research
Original – Cancer Research
Protein expression
Tumors
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Summary:Purpose Lymphocyte activation gene-3 (LAG3) is an immunosuppressive checkpoint molecule expressed on T cells. The frequency and distribution of LAG3 expression in oesophageal adenocarcinoma (EAC) is unknown. Aim of the study was the evaluation and distribution of LAG3 on tumour infiltrating lymphocytes (TILs) and correlation with clinico-pathological and molecular data. Methods We analysed tumor tissue samples using immunohistochemistry, multi-colour immunofluorescence and mRNA in-situ technology. The analyses were performed on a multi-spot tissue microarray (TMA) with 165 samples, followed by an evaluation on a single-spot TMA with 477 samples. These results were correlated with clinical and molecular tumour data. Results LAG3 expression on TILs was detectable in 10.5% on the multi-spot TMA and 11.4% on the single-spot TMA. There was a strong correlation between protein expression and mRNA expression ( p  
ISSN:0171-5216
1432-1335
DOI:10.1007/s00432-020-03295-7