Dysbiosis in intestinal microbiome linked to fecal blood determined by direct hybridization

The important physiological and pathophysiological roles of intestinal human microbiome (HMB) in human health have been emerging, owing to the access to molecular biology techniques. Herein we evaluated, for the first time, the intestinal HMB through direct hybridization approach using n-counter fle...

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Bibliographic Details
Published in:3 Biotech 2020-08, Vol.10 (8), Article 358
Main Authors: Cafiero, Concetta, Re, Agnese, Pisconti, Salvatore, Trombetti, Marina, Perri, Mariarita, Colosimo, Manuela, D’Amato, Gerardo, Gallelli, Luca, Cannataro, Roberto, Molinario, Clelia, Fazio, Alessia, Caroleo, Maria Cristina, Cione, Erika
Format: Article
Language:English
Subjects:
Agriculture
Bacteria
Bioinformatics
Biomaterials
Biotechnology
Blood
Cancer Research
Chemistry
Chemistry and Materials Science
Dysbacteriosis
Firmicutes
Hybridization
Intestine
Microbiomes
Molecular biology
rRNA 16S
Short Reports
Stem Cells
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Summary:The important physiological and pathophysiological roles of intestinal human microbiome (HMB) in human health have been emerging, owing to the access to molecular biology techniques. Herein we evaluated, for the first time, the intestinal HMB through direct hybridization approach using n-counter flex DX technology which bypasses the amplification procedure currently applied by other technologies to study the human microbiome. To this purpose, a clinical study was carried out on fecal samples, recruiting both healthy volunteers (N-FOB) and subjects positive for occult blood (P-FOB). A relevant custom panel of 79 16S rRNA target gene was engineered and 32 of them displayed a variation between the two clusters of subjects. Our findings revealed that bacteria belonging to Proteobacteria have higher distribution in P-FOB describing dysbiosis. Similarly, Bacteroidetes and Firmicutes phylum display high distribution in P-FOB. Of interest, the presence of Clostridium difficile that belongs to Firmicutes phylum displayed about 70% of low presence in N-FOB compared to P-FOB subjects. Only one bacterium belonging to the Actinobacteria phylum, the Bifidobacterium bifidum , was present.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-020-02351-w