First report of diazotrophic Brevundimonas spp. as growth enhancer and root colonizer of potato

• Published in: Scientific reports 2020-07, Vol.10 (1), p.12893-12893, Article 12893
• Main Authors: Naqqash, Tahir, Imran, Asma, Hameed, Sohail, Shahid, Muhammad, Majeed, Afshan, Iqbal, Javed, Hanif, Muhammad Kashif, Ejaz, Shaghef, Malik, Kauser Abdullah
• Format: Article
• Language: English
• Subjects: 631/326; 631/326/171; 631/326/171/1818; 631/337; 631/61; Acetic acid; Acetylene reduction; Caulobacteraceae - genetics; Caulobacteraceae - growth & development; Colonization; Confocal microscopy; Crop yield; Cultivars; Humanities and Social Sciences; Indoleacetic acid; Inoculation; Microscopy; multidisciplinary; NifH gene; Nitrogen; Nitrogen fixation; Phylogeny; Plant Roots - growth & development; Plant Roots - microbiology; Potatoes; Rhizoplane; Rhizosphere; rRNA 16S; Science; Science (multidisciplinary); Soil Microbiology; Solanum tuberosum - growth & development; Solanum tuberosum - microbiology; Solubilization; Transmission electron microscopy; Vegetables
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-69782-6

Rhizobacteria contain various plant-beneficial traits and their inoculation can sustainably increase crop yield and productivity. The present study describes the growth-promoting potential of Brevundimonas spp. isolated from rhizospheric soil of potato from Sahiwal, Pakistan. Four different putative strains TN37, TN39, TN40, and TN44 were isolated by enrichment on nitrogen-free malate medium and identified as Brevundimonas spp. based on their morphology, 16S rRNA gene sequence, and phylogenetic analyses. All strains contained nif H gene except TN39 and exhibited nitrogen fixation potential through acetylene reduction assay (ARA) except TN40. Among all, the Brevundimonas sp. TN37 showed maximum ARA and phosphate solubilization potential but none of them exhibited the ability to produce indole acetic acid. Root colonization studies using transmission electron microscopy and confocal laser scanning microscopy showed that Brevundimonas sp. TN37 was resident over the root surface of potato; forming sheets in the grooves in the rhizoplane. TN37, being the best among all was further evaluated in pot experiment using potato cultivar Kuroda in sterilized sand. Results showed that Brevundimonas sp. TN37 increased growth parameters and nitrogen uptake as compared to non-inoculated controls. Based on the results obtained in this study, it can be suggested that Brevundimonas spp. (especially TN37) possess the potential to improve potato growth and stimulate nitrogen uptake. This study is the first report of Brevundimonas spp. as an effective PGPR in potato.