Proteomic analysis identifies plasma correlates of remote ischemic conditioning in the context of experimental traumatic brain injury

Remote ischemic conditioning (RIC), transient restriction and recirculation of blood flow to a limb after traumatic brain injury (TBI), can modify levels of pathology-associated circulating protein. This study sought to identify TBI-induced molecular alterations in plasma and whether RIC would modul...

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Bibliographic Details
Published in:Scientific reports 2020-07, Vol.10 (1), p.12989-12989, Article 12989
Main Authors: Saber, Maha, Pathak, Khyati V., McGilvrey, Marissa, Garcia-Mansfield, Krystine, Harrison, Jordan L., Rowe, Rachel K., Lifshitz, Jonathan, Pirrotte, Patrick
Format: Article
Language:English
Subjects:
631/154/53
631/378
Actin
Actins - blood
Amino acids
Animals
Biomarkers - blood
Blood flow
Brain Injuries, Traumatic - blood
Brain Injuries, Traumatic - therapy
Carnosine
Disease Models, Animal
Humanities and Social Sciences
Ischemia
Ischemic Preconditioning
Male
Metabolites
Mice
multidisciplinary
Proteins
Proteomics
Righting reflex
Science
Science (multidisciplinary)
Skeletal muscle
Traumatic brain injury
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Summary:Remote ischemic conditioning (RIC), transient restriction and recirculation of blood flow to a limb after traumatic brain injury (TBI), can modify levels of pathology-associated circulating protein. This study sought to identify TBI-induced molecular alterations in plasma and whether RIC would modulate protein and metabolite levels at 24 h after diffuse TBI. Adult male C57BL/6 mice received diffuse TBI by midline fluid percussion or were sham-injured. Mice were assigned to treatment groups 1 h after recovery of righting reflex: sham, TBI, sham RIC, TBI RIC. Nine plasma metabolites were significantly lower post-TBI (six amino acids, two acylcarnitines, one carnosine). RIC intervention returned metabolites to sham levels. Using proteomics analysis, twenty-four putative protein markers for TBI and RIC were identified. After application of Benjamini–Hochberg correction, actin, alpha 1, skeletal muscle (ACTA1) was found to be significantly increased in TBI compared to both sham groups and TBI RIC. Thus, identified metabolites and proteins provide potential biomarkers for TBI and therapeutic RIC in order to monitor disease progression and therapeutic efficacy.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-69865-4