A SNP-Based Genome-Wide Association Study to Mine Genetic Loci Associated to Salinity Tolerance in Mungbean ( Vigna radiata L.)

• Published in: Genes 2020-07, Vol.11 (7), p.759
• Main Authors: Breria, Caleb Manamik, Hsieh, Ching-Hsiang, Yen, Tsair-Bor, Yen, Jo-Yi, Noble, Thomas J, Schafleitner, Roland
• Format: Article
• Language: English
• Subjects: Abiotic stress; Agricultural land; Ammonium; Beans; Cation Transport Proteins - genetics; Cereals; Chromosome 7; Chromosome 9; Gene expression; Genome, Plant; Genome-wide association studies; Genomes; Genotyping; Germplasm; Glutaredoxin; Hormones; Legumes; Physiology; Plant growth; Plant Proteins - genetics; Polymorphism, Single Nucleotide; Protein transport; Quantitative Trait Loci; Salinity; Salinity effects; Salinity tolerance; Salt; Salt Tolerance; Seed germination; Seeds; Seeds - genetics; Senescence; Single-nucleotide polymorphism; Vigna - genetics; Vigna - metabolism; Vigna radiata
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes11070759

Mungbean ( (L.) R. Wilzeck var. ) is a protein-rich short-duration legume that fits well as a rotation crop into major cereal production systems of East and South-East Asia. Salinity stress in arid areas affects mungbean, being more of a glycophyte than cereals. A significant portion of the global arable land is either salt or sodium affected. Thus, studies to understand and improve salt-stress tolerance are imminent. Here, we conducted a genome-wide association study (GWAS) to mine genomic loci underlying salt-stress tolerance during seed germination of mungbean. The World Vegetable Center (WorldVeg) mungbean minicore collection representing the diversity of mungbean germplasm was utilized as the study panel and variation for salt stress tolerance was found in this germplasm collection. The germplasm panel was classed into two agro-climatic groups and showed significant differences in their germination abilities under salt stress. A total of 5288 SNP markers obtained through genotyping-by-sequencing (GBS) were used to mine alleles associated with salt stress tolerance. Associated SNPs were identified on chromosomes 7 and 9. The associated region at chromosome 7 (position 2,696,072 to 2,809,200 bp) contains the gene , which was annotated as the ammonium transport protein (AMT). The associated region in chromosome 9 (position 19,390,227 bp to 20,321,817 bp) contained the genes and , annotated as OsGrx_S16-glutaredoxin subgroup II and dnaJ domain proteins respectively. These proteins were reported to have functions related to salt-stress tolerance.