Resuspending samples in analytical ultracentrifugation

In analytical ultracentrifugation it is often very useful to resuspend samples in situ after sedimentation experiments for further investigation. This can be achieved by manually subjecting the entire sample cell assembly to gentle motion that causes the air bubble in the sample compartment to repea...

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Bibliographic Details
Published in:Analytical biochemistry 2020-09, Vol.604, p.113771-113771, Article 113771
Main Authors: Schuck, Leonardo M., Zhao, Huaying
Format: Article
Language:English
Subjects:
Analytical ultracentrifugation
Automation, Laboratory
Laboratory automation
Sedimentation velocity
Serum Albumin, Bovine - chemistry
Specimen Handling - instrumentation
Suspensions
Thyroglobulin - chemistry
Ultracentrifugation
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Summary:In analytical ultracentrifugation it is often very useful to resuspend samples in situ after sedimentation experiments for further investigation. This can be achieved by manually subjecting the entire sample cell assembly to gentle motion that causes the air bubble in the sample compartment to repeatedly move through the solution and thereby cause convection. Here we describe a cell mixing device that can accomplish the same through axial rotation and slow rocking motion. This cell mixer is low-cost, open-source, and can be easily assembled from readily available components. It can efficiently mix multiple sample cells side-by-side and may be used with various centerpiece designs. •Resuspending samples in analytical ultracentrifugation is important but tedious.•It is part of the workflow and can reveal protein properties at high concentration.•A new low-cost open-source cell mixer is described and tested.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2020.113771