hsa‑microRNA‑411‑5p regulates proliferation, migration and invasion by targeting the hyaluronan mediated motility receptor in ovarian cancer

The mortality rate of ovarian cancer is the highest out of all gynecological malignancies worldwide. Therefore, it is important to understand the mechanisms of ovarian cancer, identify new biomarkers and develop targeted drugs. The role and molecular mechanisms of hsa-microRNA (miR)-411-5p in ovaria...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2020-09, Vol.20 (3), p.1899-1906
Main Authors: He, Fang, Zu, Dongyu, Lan, Chong, Niu, Jumin, Nie, Xiaocui
Format: Article
Language:English
Subjects:
Analysis
Biotechnology
Cancer research
Cell adhesion & migration
China
Gynecology
Health aspects
Health savings accounts
Hyaluronic acid
Infection
Kinases
Medical prognosis
Membranes
MicroRNA
MicroRNAs
Mortality
Ovarian cancer
Proteins
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Summary:The mortality rate of ovarian cancer is the highest out of all gynecological malignancies worldwide. Therefore, it is important to understand the mechanisms of ovarian cancer, identify new biomarkers and develop targeted drugs. The role and molecular mechanisms of hsa-microRNA (miR)-411-5p in ovarian cancer have not been fully elucidated. The present study investigated the ovarian cancer cell lines OVCAR-8 and SKOV3. After transfection with miRNA mimics, cell proliferation was monitored by a proliferation assay. Furthermore, cell migration was measured by a cell wound healing assay and cell invasion was measured by Matrigel invasion assays. A miRNA luciferase reporter assay was used to analyze the relationship between miRNAs and the target gene HMMR, which was then further evaluated by gene differential analysis. In the current study, hsa-mir-411-5p was identified as a miRNA regulator of the hyaluronan mediated motility receptor, which negatively regulated the activity of ERK1/2 and ultimately inhibited ovarian cancer cell proliferation and motility. Although hsa-mir-411-5p may have different roles in other types of cancer, the present study suggested that miR-411-5p functions as a negative tumor regulator in ovarian cancer cells, displaying the potential of miR-411-5p as a biomarker for ovarian cancer.
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2020.8899