Insights into Metabolic Reactions of Semi-Dwarf, Barley Brassinosteroid Mutants to Drought

• Published in: International journal of molecular sciences 2020-07, Vol.21 (14), p.5096
• Main Authors: Gruszka, Damian, Pociecha, Ewa, Jurczyk, Barbara, Dziurka, Michał, Oliwa, Jakub, Sadura, Iwona, Janeczko, Anna
• Format: Article
• Language: English
• Subjects: Abnormalities; Accumulation; Agricultural production; Antioxidants; Ascorbate Peroxidases - genetics; Ascorbate Peroxidases - metabolism; Ascorbic acid; Barley; Biosynthesis; Brassinosteroids; Brassinosteroids - metabolism; Catalase; Catalase - genetics; Catalase - metabolism; Climate change; Cultivars; Drought; Droughts; Environmental conditions; Enzymatic activity; Enzymes; Fructose - metabolism; Genes, Plant; Glucose; Glucose - metabolism; Homeostasis; Hordeum - genetics; Hordeum - growth & development; Hordeum - metabolism; Hordeum vulgare; Hydrogen Peroxide - metabolism; L-Ascorbate peroxidase; Metabolism; Mutation; Peroxidase; Physiology; Plant growth; Plant Growth Regulators - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Ribulose-Bisphosphate Carboxylase - genetics; Ribulose-Bisphosphate Carboxylase - metabolism; Stress, Physiological - genetics; Stress, Physiological - physiology; Sucrose; Superoxide dismutase; Superoxide Dismutase-1 - genetics; Superoxide Dismutase-1 - metabolism; Transcription
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21145096

The roles of endogenous brassinosteroids (BRs) in the modulation of reaction to drought and genetic regulation of this process are still obscure. In this study, a multidirectional analysis was performed on semi-dwarf barley ( ) Near-Isogenic Lines (NILs) and the reference cultivar "Bowman" to get insights into various aspects of metabolic reaction to drought. The NILs are defective in BR biosynthesis or signaling and displayed an enhanced tolerance to drought. The BR metabolism perturbations affected the glucose and fructose accumulation under the control and stress conditions. The BR metabolism abnormalities negatively affected the sucrose accumulation as well. However, during drought, the BR-deficient NILs accumulated higher contents of sucrose than the "Bowman" cultivar. Under the control conditions, accumulation of transcripts encoding antioxidant enzymes ascorbate peroxidase (HvAPX) and superoxide dismutase (HvSOD) was BR-dependent. However, during drought, the accumulation of transcript was BR-dependent, whereas accumulations of transcripts encoding catalase (HvCAT) and HvSOD were not affected by the BR metabolism perturbations. The obtained results reveal a significant role of BRs in regulation of the HvAPX and HvCAT enzymatic activities under control conditions and the HvAPX and HvSOD activities during physiological reactions to drought.