The Role of miR-375-3p and miR-200b-3p in Gastrointestinal Stromal Tumors

• Published in: International journal of molecular sciences 2020-07, Vol.21 (14), p.5151
• Main Authors: Gyvyte, Ugne, Lukosevicius, Rokas, Inciuraite, Ruta, Streleckiene, Greta, Gudoityte, Greta, Bekampyte, Justina, Valentini, Serena, Salteniene, Violeta, Ruzgys, Paulius, Satkauskas, Saulius, Zviniene, Kristina, Kupcinskas, Juozas, Skieceviciene, Jurgita
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions; Apoptosis; Apoptosis - genetics; Carcinogenesis; Carcinogens; Cell adhesion & migration; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Cell Survival - genetics; Cell viability; Deregulation; DNA-Binding Proteins - metabolism; Down-Regulation; Epidermal growth factor receptors; ErbB Receptors - genetics; ErbB Receptors - metabolism; Gastric cancer; Gastrointestinal cancer; Gastrointestinal Neoplasms - genetics; Gastrointestinal Neoplasms - metabolism; Gastrointestinal Stromal Tumors - genetics; Gastrointestinal Stromal Tumors - metabolism; Gene expression; Gene Expression Regulation, Neoplastic - genetics; Humans; Kinases; Liver cancer; Mesenchyme; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; mRNA; Mutation; Proteins; Studies; Transcription Factors - metabolism; Tumorigenesis; Tumors
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21145151

Deregulated microRNA (miRNA) expression profiles and their contribution to carcinogenesis have been observed in virtually all types of human cancer. However, their role in the pathogenesis of rare mesenchymal gastrointestinal stromal tumors (GISTs) is not well defined, yet. In this study, we aimed to investigate the role of two miRNAs strongly downregulated in GIST-miR-375-3p and miR-200b-3p-in the pathogenesis of GIST. To achieve this, miRNA mimics were transfected into GIST-T1 cells and changes in the potential target gene mRNA and protein expression, as well as alterations in cell viability, migration, apoptotic cell counts and direct miRNA-target interaction, were evaluated. Results revealed that overexpression of miR-375-3p downregulated the expression of KIT mRNA and protein by direct binding to KIT 3'UTR, reduced GIST cell viability and migration rates. MiR-200b-3p lowered expression of ETV1 protein, directly targeted and lowered expression of EGFR mRNA and protein, and negatively affected cell migration rates. To conclude, the present study identified that miR-375-3p and miR-200b-3p have a tumor-suppressive role in GIST.