Vertical Transmission of Zika Virus in Aedes aegypti Produces Potentially Infectious Progeny

• Published in: The American journal of tropical medicine and hygiene 2020-08, Vol.103 (2), p.876-883
• Main Authors: Comeau, Genevieve, Zinna, Robert A, Scott, Taylor, Ernst, Kacey, Walker, Kathleen, Carrière, Yves, Riehle, Michael A
• Format: Article
• Language: English
• Subjects: Aedes - genetics; Aedes - virology; Animal reproduction; Animals; Disease transmission; Female; Infectious Disease Transmission, Vertical; Mosquito Vectors - virology; Mosquitoes; Saliva - virology; Vectors (Biology); Viral Envelope Proteins - genetics; Viral Load; Virulence; Zika virus; Zika Virus - genetics; Zika Virus - pathogenicity; Zika Virus Infection - transmission
• ISSN: 0002-9637; 1476-1645
• DOI: 10.4269/ajtmh.19-0698

Vertical transmission, or pathogen transfer from female to offspring, can facilitate the persistence of emerging arboviruses, such as Zika virus (ZIKV), through periods of low horizontal transmission or adverse environmental conditions. We aimed at determining the rate of vertical transmission for ZIKV in its principal vector, , and the vector competence of vertically infected progeny. females that consumed a blood meal provisioned with ZIKV were maintained under three temperature conditions (27°C, 30°C, and 33°C) following the infectious blood meal and allowed to complete three reproductive cycles. The overall vertical transmission rate was 6.5% (95% CI = 3.9-9.9). Vertical transmission of ZIKV was observed across all temperature conditions and virus detected in adult progeny up to 2 weeks postemergence. In total, 3.4% (95% CI = 1.6-6.2) of adult progeny produced saliva with ZIKV, indicating their vector competence. These results suggest the virus may be maintained in populations without a vertebrate host for short periods.