Responses of Primary Afferent Fibers to Acupuncture-Like Peripheral Stimulation at Different Frequencies: Characterization by Single-Unit Recording in Rats

• Published in: Neuroscience bulletin 2020-08, Vol.36 (8), p.907-918
• Main Authors: Huo, Ran, Han, Song-Ping, Liu, Feng-Yu, Shou, Xiao-Jing, Liu, Ling-Yu, Song, Tian-Jia, Zhai, Fu-Jun, Zhang, Rong, Xing, Guo-Gang, Han, Ji-Sheng
• Format: Article
• Language: English
• Subjects: Acupuncture; Anatomy; Anesthesiology; Biomedical and Life Sciences; Biomedicine; Human Physiology; Neurology; Neurophysiology; Neurosciences; Original; Original Article; Pain Medicine
• ISSN: 1673-7067; 1995-8218
• DOI: 10.1007/s12264-020-00509-3

The pain-relieving effect of acupuncture is known to involve primary afferent nerves (PANs) via their roles in signal transmission to the CNS. Using single-unit recording in rats, we characterized the generation and transmission of electrical signals in Aβ and Aδ fibers induced by acupuncture-like stimuli. Acupuncture-like signals were elicited in PANs using three techniques: manual acupuncture (MAc), emulated acupuncture (EAc), and electro-acupuncture (EA)-like peripheral electrical stimulation (PES). The discharges evoked by MAc and EAc were mostly in a burst pattern with average intra-burst and inter-burst firing rates of 90 Hz and 2 Hz, respectively. The frequency of discharges in PANs was correlated with the frequency of PES. The highest discharge frequency was 246 Hz in Aβ fibers and 180 Hz in Aδ fibers. Therefore, EA in a dense-disperse mode (at alternating frequency between 2 Hz and 15 Hz or between 2 Hz and 100 Hz) best mimics MAc. Frequencies of EA output >250 Hz appear to be obsolete for pain relief.