Mast cell degranulation and histamine release during A/H5N1 influenza infection in influenza-sensitized mice

Here we evaluate the role of mast cells in infection with influenza A/H5N1 virus in immunized mice. CBA mice were immunized intramuscularly with formalin-inactivated A/Vietnam/1194/2004 (H5N1)NIBRG-14 (H5N1). Serum samples were obtained on days 7, 12, 14, 21 after immunization. At day 14, the mice w...

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Bibliographic Details
Published in:Life sciences (1973) 2020-10, Vol.258, p.118230-118230, Article 118230
Main Authors: Desheva, Yulia, Mamontov, Andrey, Petkova, Nadezhda, Karev, Vadim, Nazarov, Peter
Format: Article
Language:English
Subjects:
Animals
Antihistamines
Avian flu
CD117
Cell Degranulation - physiology
Chick Embryo
Chlorocebus aethiops
Degranulation
Histamine
Histamine Release - physiology
Immune complexes
Immune response
Immune system
Immunization
Infections
Influenza
Influenza A
Influenza A Virus, H5N1 Subtype
Influenza infection
Influenza vaccination
Lethality
Lungs
Mast cells
Mast Cells - pathology
Mast Cells - physiology
Mast Cells - virology
Mice
Orthomyxoviridae Infections - metabolism
Orthomyxoviridae Infections - pathology
Pathogenesis
Vaccination
Vaccines
Vero Cells
Viruses
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Summary:Here we evaluate the role of mast cells in infection with influenza A/H5N1 virus in immunized mice. CBA mice were immunized intramuscularly with formalin-inactivated A/Vietnam/1194/2004 (H5N1)NIBRG-14 (H5N1). Serum samples were obtained on days 7, 12, 14, 21 after immunization. At day 14, the mice were infected intranasally with the A/Indonesia/5/2005 (H5N1)IDCDC-RG2 (H5N1) influenza virus with half of the animals receiving a mixture of the antihistamines. 67% of the vaccinated mice were protected from the lethality compared to 43% in the PBS-immunized group. Administration of antihistamines increased survival up to 85%–95%. Immunohistochemical examination using CD117 staining of the lungs demonstrated a larger quantity of activated mast cells after infection of immunized mice compared to mock-immunized mice. This was correlated to increased histamine level in the lungs and blood. Our experimental results suggest the involvement of mast cells and the histamine they produce in the pathogenesis of influenza infection in case of incomplete formation of the immune response to vaccination and mismatch of the vaccine and infection influenza viruses.
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2020.118230