Exploration of the effects of goose TCs on GCs at different follicular stages using a co-culture model

Granulosa cells (GCs) play a critical role in follicular development, which cannot be separated from the assistance of theca cells (TCs). In the present study, we used a transwell system to develop three stages of goose GCs in vitro mono-culture and co-culture models, and we analyzed the morphology,...

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Bibliographic Details
Published in:Bioscience reports 2020-08, Vol.40 (8)
Main Authors: Gan, Xiang, Wang, Yushi, Gao, Shanyan, Chen, Xi, Hu, Shenqiang, Wang, Jiwen, Hu, Jiwei, Li, Liang, Han, Chunchun
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Cell Communication
Cell Cycle, Growth & Proliferation
Cell Proliferation
Cells, Cultured
Coculture Techniques
Female
Geese
Gene Expression & Regulation
Gene Expression Regulation, Developmental
Gonadal Steroid Hormones - biosynthesis
Granulosa Cells - metabolism
Lipogenesis
Molecular Interactions
Ovarian Follicle - cytology
Signal Transduction
Theca Cells - metabolism
Time Factors
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Summary:Granulosa cells (GCs) play a critical role in follicular development, which cannot be separated from the assistance of theca cells (TCs). In the present study, we used a transwell system to develop three stages of goose GCs in vitro mono-culture and co-culture models, and we analyzed the morphology, activity, intracellular lipid content and the expression of core genes involved in de novo lipogenesis (DNL), steroidogenesis, proliferation and apoptosis of the GCs. In the co-culture group, the activity of all three stages of GCs showed significant (P
ISSN:0144-8463
1573-4935
DOI:10.1042/BSR20200445