Enzymatically synthesized glycogen inhibited degranulation and inflammatory responses through stimulation of intestine

The patients of type I allergic diseases were increased in the developed countries. Recently, many studies have focused on food factors with anti-allergic activities. Enzymatically synthesized glycogen, a polysaccharide with a multi-branched α-1,4 and α-1,6 linkages, is a commercially available prod...

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Published in:Journal of Clinical Biochemistry and Nutrition 2020, Vol.67(1), pp.67-73
Main Authors: Yoshioka, Yasukiyo, Inoue, Masako, Yoshioka, Hiroko, Kitakaze, Tomoya, Furuyashiki, Takashi, Abe, Naoki, Ashida, Hitoshi
Format: Article
Language:English
Subjects:
AKT protein
Allergic diseases
anti-allergy
anti-inflammation
Antigens
Caco-2 cells
Cell culture
Degranulation
Developed countries
enzymatically synthesized glycogen
Food
Food allergies
Glycogen
Glycogens
Immunostimulation
Inflammation
Interleukin 6
Intestine
Kinases
Original
Phospholipase
Phospholipase C
Phosphorylation
Polysaccharides
RBL-2H3 cells
Starch
Synthesis
Tumor necrosis factor-α
Tyrosine
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Summary:The patients of type I allergic diseases were increased in the developed countries. Recently, many studies have focused on food factors with anti-allergic activities. Enzymatically synthesized glycogen, a polysaccharide with a multi-branched α-1,4 and α-1,6 linkages, is a commercially available product from natural plant starch, and has immunostimulation activity. However, effect of enzymatically synthesized glycogen on the anti-allergic activity was unclear yet. In this study, we investigated that enzymatically synthesized glycogen inhibited allergic and inflammatory responses using a co-culture system consisting of Caco-2 and RBL-2H3 cells. Enzymatically synthesized glycogen inhibited antigen-induced β-hexosaminidase release and production of TNF-α and IL-6 in RBL-2H3 cells in the co-culture system. Furthermore, enzymatically synthesized glycogen inhibited antigen-induced phosphorylation of tyrosine kinases, phospholipase C γ1/2, mitogen-activated protein kinases and Akt. Anti-allergic and anti-inflammatory activities of enzymatically synthesized glycogen were indirect action through stimulating Caco-2 cells, but not by the direct interaction with RBL-2H3 cells, because enzymatically synthesized glycogen did not permeate Caco-2 cells. These findings suggest that enzymatically synthesized glycogen is an effective food ingredient for prevention of type I allergy through stimulating the intestinal cells.
ISSN:0912-0009
1880-5086
DOI:10.3164/jcbn.20-33