Assessment of GSTO1 (A140D) and GSTO2 (N142D) Gene Polymorphisms in Iranian Women with Polycystic Ovarian Syndrome

• Published in: Reports of biochemistry and molecular biology 2020-04, Vol.9 (1), p.8-13
• Main Authors: Miraghaee, Seyyed Shahram, Sohrabi, Maryam, Jalili, Cyrus, Bahrehmand, Fariborz
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 2322-3480; 2322-3480
• DOI: 10.29252/rbmb.9.1.8

Glutathione S-transferases (GSTs) protect cells from oxidative stress (OS). In humans, the GST omega class contains two expressed genes, and . Because OS is involved in the pathogenesis of polycystic ovary syndrome (PCOS), the aim of this study was to investigate the relationship between A140D (rs4925) and N142D (rs156697) polymorphisms in PCOS patients. 175 PCOS patients and 161 healthy controls were selected among women in Kermanshah province, Iran. and were genotyped using allele-specific PCR (AS-PCR) and PCR-RFLP, respectively. For , the DD genotype and the D allele led to 2.17- ( = 0.02) and 1.5-fold (P= 0.01) increases, respectively, in the odds ratios for PCOS. No significant difference was found between control and patient groups for the N142D genotype or allele frequency. GSTO1 and GSTO2 genotype interaction analysis showed that individuals with the AD or DD genotypes and the GSTO2 NN or DN genotypes had a 1.53-fold ( = 0.007) increase in PCOS risk over AA and GSTO2 DD individuals. The A140D polymorphism is a risk factor for PCOS.