Pharyngeal carriage rates of Neisseria meningitidis in health care professionals at a tertiary university pediatric hospital

• Published in: European journal of clinical microbiology & infectious diseases 2020-09, Vol.39 (9), p.1703-1709
• Main Authors: Steurer, Lisa-Maria, Hetzmannseder, Mathias, Willinger, Birgit, Starzengruber, Peter, Mikula, Claudia, Kormann-Klement, Andrea, Weber, Michael, Berger, Angelika, Grill, Agnes
• Format: Article
• Language: English
• Subjects: Adolescent; Adolescents; Adult; Age; Austria - epidemiology; Biomedical and Life Sciences; Biomedicine; Carrier State - epidemiology; Carrier State - microbiology; Carrier State - transmission; Colonization; Complications; Cross-Sectional Studies; Disease control; Disease transmission; Female; Gene sequencing; Genomes; Genotypes; Health care; Health Personnel; Hospitals, Pediatric; Humans; Infants; Internal Medicine; Male; Medical Microbiology; Medical personnel; Meningococcal Infections - epidemiology; Meningococcal Infections - microbiology; Meningococcal Infections - transmission; Middle Aged; Neisseria meningitidis; Neisseria meningitidis - genetics; Neisseria meningitidis - isolation & purification; Occupational Diseases - epidemiology; Occupational Diseases - microbiology; Original; Original Article; Pediatrics; Pharynx; Pharynx - microbiology; Prevalence; Risk analysis; Risk factors; Surveys and Questionnaires; Universities; Whole genome sequencing; Young Adult
• ISSN: 0934-9723; 1435-4373
• DOI: 10.1007/s10096-020-03894-9

Pharyngeal carriage is the reservoir for Neisseria meningitidis in the population and the first step in disease transmission. Especially in young infants and adolescents, N. meningitidis can cause serious invasive infection with high fatality rates and high rates of long-term sequelae among survivors. The aim of this study was to determine N. meningitidis colonization rates in asymptomatic health care professionals at a tertiary university pediatric hospital and to identify risk factors for carriage. This cross-sectional meningococcal carriage survey was conducted between April and October 2018 at the Medical University of Vienna. Individuals working as nurses, pediatricians, or medical students were enrolled. Oropharyngeal swabs were directly plated onto selective agar plates and conventional culture was used for bacterial identification. Meningococcal isolates were further characterized using whole-genome sequencing. A total of 437 oropharyngeal specimens were collected. Overall, meningococcal carriage prevalence was 1.14% (5/437), with 0.7% (3/437) for capsular genotype B, and 0.5% (2/437) for capsular genotype W. Mean age of carriers was significantly lower than of non-carriers (24.2 vs. 35.8; p  = 0.004). The highest carriage rate of 4.4% (4/91) was found in the age group 18–25. Carriage was negatively associated with age and timespan working in pediatrics. This is the first study evaluating the prevalence of Neisseria meningitidis carriage in health care professionals working in Pediatrics and Adolescent Medicine. Carriage was in general lower than expected for all age groups, implicating a low risk of meningococcal transmission via this population.