Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic

The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment...

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Bibliographic Details
Published in:International journal of environmental research and public health 2020-07, Vol.17 (15), p.5508
Main Authors: Mlejnkova, Hana, Sovova, Katerina, Vasickova, Petra, Ocenaskova, Vera, Jasikova, Lucie, Juranova, Eva
Format: Article
Language:English
Subjects:
Betacoronavirus - isolation & purification
Communication
Coronavirus Infections - epidemiology
Coronavirus Infections - virology
Coronaviruses
COVID-19
Czech Republic - epidemiology
Disease transmission
Efficiency
Epidemics
Epidemiology
Feces
Feces - virology
Genomes
Humans
Infections
Middle East respiratory syndrome
Pandemics
Pneumonia, Viral - epidemiology
Pneumonia, Viral - virology
Polymerase chain reaction
Population
Process controls
Real-Time Polymerase Chain Reaction
Respiratory diseases
Reverse transcription
RNA, Viral
SARS-CoV-2
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Surveillance
Viruses
Wastewater - virology
Wastewater treatment
Wastewater treatment plants
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Summary:The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population.
ISSN:1660-4601
1661-7827
1660-4601
DOI:10.3390/ijerph17155508