Role of N , N -Dimethylglycine and Its Catabolism to Sarcosine in Chromohalobacter salexigens DSM 3043

• Published in: Applied and environmental microbiology 2020-08, Vol.86 (17)
• Main Authors: Yang, Ting, Shao, Ya-Hui, Guo, Li-Zhong, Meng, Xiang-Lin, Yu, Hao, Lu, Wei-Dong
• Format: Article
• Language: English
• Subjects: Adenine; Aerobic conditions; Catabolism; Chromohalobacter - genetics; Chromohalobacter - metabolism; Chromohalobacter salexigens; Complementation; Dimethylglycine; E coli; Ectoine; Enzymatic activity; Enzyme activity; Enzymes; Flavin; Flavin-adenine dinucleotide; Gene disruption; Genes; Genes, Bacterial; Genetic Complementation Test; Glycine; Glycine betaine; Growth rate; Mutants; NAD; NADP; NMR; Nuclear magnetic resonance; Physiology; Sarcosine; Sarcosine - analogs & derivatives; Sarcosine - metabolism; Substrates; Trehalose
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.01186-20

DSM 3043 can grow on , -dimethylglycine (DMG) as the sole C, N, and energy source and utilize sarcosine as the sole N source under aerobic conditions. However, little is known about the genes and enzymes involved in the conversion of DMG to sarcosine in this strain. In the present study, gene disruption and complementation assays indicated that the , , , and genes are responsible for DMG degradation to sarcosine. The gene heterologously expressed in was proven to encode an unusual DMG dehydrogenase (DMGDH). The enzyme, existing as a monomer of 79 kDa with a noncovalently bound flavin adenine dinucleotide, utilized both DMG and sarcosine as substrates and exhibited dual coenzyme specificity, preferring NAD to NADP The optimum pH and temperature of enzyme activity were determined to be 7.0 and 60°C, respectively. Kinetic parameters of the enzyme toward its substrates were determined accordingly. Under high-salinity conditions, the presence of DMG inhibited growth of the wild type and induced the production and accumulation of trehalose and glucosylglycerate intracellularly. Moreover, exogenous addition of DMG significantly improved the growth rates of the four DMG mutants ( , , , and ) incubated at 37°C in S-M63 synthetic medium with sarcosine as the sole N source. C nuclear magnetic resonance ( C-NMR) experiments revealed that not only ectoine, glutamate, and -acetyl-2,4-diaminobutyrate but also glycine betaine (GB), DMG, sarcosine, trehalose, and glucosylglycerate are accumulated intracellularly in the four mutants. Although , -dimethylglycine (DMG) dehydrogenase (DMGDH) activity was detected in cell extracts of microorganisms, the genes encoding microbial DMGDHs have not been determined until now. In addition, to our knowledge, the physiological role of DMG in moderate halophiles has never been investigated. In this study, we identified the genes involved in DMG degradation to sarcosine, characterized an unusual DMGDH, and investigated the role of DMG in DSM 3043 and its mutants. Our results suggested that the conversion of DMG to sarcosine is accompanied by intramolecular delivery of electrons in DMGDH and intermolecular electron transfer between DMGDH and other electron acceptors. Moreover, an unidentified methyltransferase catalyzing the production of glycine betaine (GB) from DMG but sharing no homology with the reported sarcosine DMG methyltransferases was predicted to be present in the cells. The results of this study expand our understanding of the