A novel immunochromatographic strips assay for rapid and simple detection of systemic lupus erythematosus

• Published in: Scientific reports 2020-08, Vol.10 (1), p.14178-14178, Article 14178
• Main Authors: Sun, Yuhan, Li, Zhi, Liang, Wei, Zhang, Yanlong, Song, Wanli, Song, Jiazhe, Xue, Kai, Wang, Meiling, Sun, Wenying, Gu, Jianguo, Li, Ming, Li, Wenzhe
• Format: Article
• Language: English
• Subjects: 631/45; 692/53; Adolescent; Adult; Aged; Animals; Antibodies, Antinuclear - blood; Aspergillus oryzae; Autoimmune diseases; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Biomarkers; Chlamydia; Chromatography, Affinity; Enzyme-Linked Immunosorbent Assay; Female; Fluorescent Antibody Technique, Indirect; Fucose - metabolism; Fucosyltransferases - deficiency; Fucosyltransferases - metabolism; Genomes; Glycosylation; Gold; Gold Colloid; Humanities and Social Sciences; Humans; Immunoblotting; Immunoglobulin G - blood; Immunoglobulin G - chemistry; Immunosuppression; Infections; Laboratory tests; Lupus; Lupus Erythematosus, Systemic - blood; Lupus Erythematosus, Systemic - diagnosis; Male; Mice, Knockout; Middle Aged; multidisciplinary; Pathogens; Phascolarctidae; Plant Lectins; Protein Processing, Post-Translational; Reagent Strips; Recombinant Fusion Proteins; Science; Science (multidisciplinary); Specific Pathogen-Free Organisms; Systemic lupus erythematosus; Viremia; Virulence; Young Adult
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-71137-0

Systemic lupus erythematosus (SLE) is a complex multi-system autoimmune disease. Detection of anti-nuclear antibodies (ANA) is fundamental for the diagnosis of SLE. In the present study, we found that the level of core fucosylation catalyzed by α1,6-fucosyltransferase (Fut8) is markedly up-regulated on immunoglobulin G (IgG) in the sera of SLE patients detected by Aspergillus oryzae lectin (AOL) blot. In sandwich Dot enzyme-linked immunosorbent assay (Dot-ELISA), the core fucosylation level was also found significantly increased in the sera from SLE patients with a higher ANA titer. To establish a rapid and sensitive laboratory test for the diagnosis of SLE, we prokaryotically expressed AOL and C3-D1-C3-D2-C3 of protein G (SpG3), and generate AOL-conjugated colloid gold immunochromatographic strips (ICS). The detection limit of core fucosylated IgG was 10 μg/mL for AOL-conjugated colloid gold ICS. As well as indirect immunofluorescence, the AOL-conjugated colloid gold ICS showed reliable results in the serum of 39 SLE patients. Our results indicated that the AOL-conjugated colloid gold ICS could serve as a rapid test for the detection of ANA and suspected cases of SLE.