USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer

To investigate the clinical significance of ubiquitin-specific peptidase 19 (USP19) expression in gastric cancer (GC) compared with that in normal tissues and gastric cell lines. USP19 protein expression was analyzed in 212 paired GC samples using immunohistochemical staining. Quantitative real-time...

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Bibliographic Details
Published in:OncoTargets and therapy 2020-01, Vol.13, p.8495-8510
Main Authors: Dong, Zhe, Guo, Shuai, Wang, Yue, Zhang, Jun, Luo, Haojie, Zheng, Guoliang, Yang, Dong, Zhang, Tao, Yan, Liucun, Song, Li, Liu, Kejia, Sun, Zhe, Meng, Xiangyu, Zheng, Zhichao, Zhang, Jianjun, Zhao, Yan
Format: Article
Language:English
Subjects:
Antibodies
Apoptosis
Biomarkers
Caspase-3
Cell adhesion & migration
Cell division
Cell proliferation
Enzymatic activity
Enzymes
Gastric cancer
Gelatin
Gelatinase A
Gelatinase B
Genomes
Guanylate cyclase
Immunohistochemistry
Medical prognosis
Metastases
Metastasis
Microscopy
Original Research
Peptidase
Proteins
Surgery
Tumorigenesis
Tumors
Ubiquitin
Western blotting
Wound healing
Xenografts
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Summary:To investigate the clinical significance of ubiquitin-specific peptidase 19 (USP19) expression in gastric cancer (GC) compared with that in normal tissues and gastric cell lines. USP19 protein expression was analyzed in 212 paired GC samples using immunohistochemical staining. Quantitative real-time PCR (qRT-PCR) and Western blotting were used to detect the level of USP19 in gastric cell lines. The biological functions of USP19 were investigated by MTT assay, colony-forming assay, wound healing assay and gelatin zymography, and apoptotic cells were detected by immunohistochemistry assays in SGC7901 xenograft models. USP19 expression was significantly increased in GC cells and tissues, and the high level of USP19 expression was positively correlated with the Lauren's classification and poor prognosis. Moreover, USP19 was identified as a novel independent prognostic biomarker in GC patients. Enhanced USP19 expression promoted GC cell proliferation and metastasis through reduced cleaved caspase-3 and increased MMP2/MMP9 expression and promoted enzyme activity in the study, and verified the results through The Cancer Genome Atlas (TCGA) and bioinformatic websites from the Kaplan-Meier plotter (http://kmplot.com) and GEPIA (http://gepia2.cancer-pku.cn.). Our study suggests that USP19 promoted tumor progression by inducing MMP2/MMP9 expression and related enzyme activity. Hence, USP19 may be a valuable prognostic predictor for GC, and the USP19-MMP2/MMP9 axis could serve as a therapeutic target.
ISSN:1178-6930
1178-6930
DOI:10.2147/OTT.S240543