Human plasma protein corona decreases the toxicity of pillar-layer metal organic framework

• Published in: Scientific reports 2020-09, Vol.10 (1), p.14569, Article 14569
• Main Authors: Jafari, Samira, Izadi, Zhila, Alaei, Loghman, Jaymand, Mehdi, Samadian, Hadi, Kashani, Vali ollah, Derakhshankhah, Hossein, Hayati, Payam, Noori, Foad, Mansouri, Kamran, Moakedi, Faezeh, Janczak, Jan, Soltanian Fard, Mohammad Jaafar, Fayaz bakhsh, Nozar
• Format: Article
• Language: English
• Subjects: 639/301; 639/301/54; 639/301/54/1754; Breast Neoplasms - drug therapy; Breast Neoplasms - pathology; Cell culture; Cell Proliferation; Computer applications; Copper; Drug Delivery Systems; Female; Fluorescence; Gel electrophoresis; Genomes; Humanities and Social Sciences; Humans; Infrared spectroscopy; Irradiation; Mathematical models; MCF-7 Cells; Metabolic pathways; Metabolism; Metal-Organic Frameworks - chemistry; Metal-Organic Frameworks - pharmacology; multidisciplinary; Ordinary differential equations; Protein Corona - chemistry; Science; Science (multidisciplinary); Serum Albumin, Human - pharmacology; Serum Globulins - pharmacology; Sodium lauryl sulfate; Stochasticity; X-ray diffraction
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-71170-z

This scenario was designed to investigate the protein corona pattern on the pillar-layer surface of a Cu-based metal–organic framework (MOF) in human plasma. The [Cu(L)(L / )].1.3DMA (MOF-1) {L = 4, 4 / -bipyridine and L /  = 5-aminoisophthalic acid}, was synthesized through the sonochemical irradiation approach as well as characterized by various techniques like scanning electron microscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction and single-crystal X-ray diffraction. The space group was determined to be an orthorhombic space group ( Pbam ) by single-crystal X-ray diffraction. Single-crystal X-ray analyses on MOF-1 showed that Cu +2 ion was 6-coordinated. Besides, to study and clarify interactions between MOFs and biological milieu, human whole blood plasma was selected as a model. Fluorescence spectroscopy and SDS-PAGE techniques were employed to explore quantitative and qualitative in situ characterization of protein corona as well. Furthermore, cell viability in a cancerous cell lines was evaluated by MTT assay in the presence and absence of the corona. The results from SDS-PAGE illustrated that the most adsorbed quantity among plasma proteins belongs to fibrinogen (α, β and γ chains), and this protein showed the maximum frequency on the MOF-1s surface, so the possible interactions of MOF-1s with fibrinogen also studied using fluorescence spectroscopy and corresponding data were plotted. According to the obtained data from MTT assay, these structures have concentration-dependent toxicity. In brief, based on the obtained data in the current study, the designed MOF can be introduced as a new desirable carrier for drug/gen delivery after further prerequisite assessments.