Hybrid Nanotopographical Surfaces Obtained by Biomimetic Mineralization of Statherin-Inspired Elastin-Like Recombinamers

• Published in: Advanced healthcare materials 2014-10, Vol.3 (10), p.1638-1647
• Main Authors: Li, Yuping, Chen, Xi, Ribeiro, Artur J., Jensen, Eric D., Holmberg, Kyle V., Rodriguez-Cabello, J. Carlos, Aparicio, Conrado
• Format: Article
• Language: English
• Subjects: 3T3 Cells; Amino Acid Sequence; Animals; Biochemistry; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biological and medical sciences; biomimetic mineralization; Biomimetics - methods; Cell Adhesion - drug effects; Cell Line; Cell physiology; Cell Proliferation - drug effects; Coating; Control surfaces; Cues; Elastin - chemistry; elastin-like recombinamers; Fundamental and applied biological sciences. Psychology; Humans; implant surfaces; Medical sciences; Mice; Mineralization; Mineralization, calcification; Molecular and cellular biology; Molecular Sequence Data; Nanostructure; Nanostructures - chemistry; Nanostructures - ultrastructure; nanotopography; Orthopedics; Osteoblasts - cytology; Osteoblasts - drug effects; Osteocalcin - metabolism; Recombinant Proteins - chemistry; Saliva - chemistry; Salivary Proteins and Peptides - chemistry; statherin; Stem cells; Surface Properties; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgical implants; Technology. Biomaterials. Equipments
• ISSN: 2192-2640; 2192-2659
• DOI: 10.1002/adhm.201400015

Modification of surfaces mimicking unique chemical and physical features of mineralized tissues is of major interest for obtaining biomaterials for replacing and regenerating biological tissues. Here, human salivary statherin‐inspired genetically engineered recombinamers (ELRs, HSS) on biomedical surfaces regulates mineralization to form an amorphous‐calcium‐phosphate (ACP) layer that reproduces the original substrate nanotopography. The HSS‐ELRs carry a statherin‐derived peptide with high affinity to tooth enamel. They are tethered to nanorough surfaces and mineralized using an enzyme‐directed process. A homogeneous layer of ACP‐minerals forms on HSS‐coated surfaces retaining the original nanotopography of the substrate. In contrast, biomineralization of control surfaces results in uncontrolled growth of minerals. This suggest the statherin‐inspired ELRs have ability to induce and control growth of the minerals on the biofunctional surfaces. Likely, the HSS‐ELR coating have similar bioactivity to that of statherin in human saliva. The hybrid nanorough surfaces improve adhesion and differentiation of preosteoblasts and show potential for dental and orthopedic implants integration. This method enables the combination and tailoring of nanotopographical and biochemical cues to design functionalized surfaces to investigate and potentially direct the stem cell fate. Hybrid nanorough surfaces are obtained by coating‐etched titanium with statherin‐inspired ELRs that controls amorphous calcium mineralization through a biomimetic enzyme‐directed process. This method enables the combination and tailoring of physical and biochemical cues to obtain dental and orthopedic implant surfaces and to design functionalized surfaces to investigate and potentially direct the stem cell fate.