Analysis of bacterial communities of infected primary teeth in a Mexican population

• Published in: Medicina oral, patología oral y cirugía bucal patología oral y cirugía bucal, 2020-09, Vol.25 (5), p.e668-e674
• Main Authors: Salas-López, EK, Casas-Flores, S., López-Lozano, NE, Layseca-Espinosa, E., García-Sepúlveda, CA, Niño-Moreno, Pd, Pozos-Guillén, A.
• Format: Article
• Language: English
• Subjects: Dentistry
• ISSN: 1698-6946; 1698-4447; 1698-6946
• DOI: 10.4317/medoral.23689

BACKGROUNDThe objective of this study was to describe the bacterial communities associated with pediatric patients with endodontic infections of temporal teeth by targeting the 16S rRNA gene using pyrosequencing. MATERIAL AND METHODSMicrobiological samples were obtained from the lower primary molars of thirteen 13 pediatric patients with dental infections. An aspiration method for microbiological sampling was used. The identification of microbiota employing the pyrosequencing method by targeting the 16S gene was performed. RESULTSRibosomal 16S RNA gene sequences were amplified, obtaining a total of 16,182 sequences from 13 primary infected molars (13 different individuals) by pyrosequencing. Bacteroidetes phyla (35.15%) were the most abundant followed by Firmicutes (33.3%) and Fusobacteria (10.05%); the presence of specific pathogenic bacteria was determined as well. CONCLUSIONSThe infected root canal of primary teeth contains a high diversity of anaerobic bacteria, and Bacteroidetes, Firmicutes, and Fusobacteria phyla were the most abundant; Prevotella and Streptococcus genera were the most prevalent.