TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers

Background and Aim: Invasion and metastasis are critical events in papillary thyroid carcinoma (PTC) progression. Protein markers specific to this process may avoid over-treatment and urgently needed. Methods: TMT-labeled mass spectrometry-based proteomics were carried out on PTC and invasive phenot...

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Bibliographic Details
Published in:Journal of Cancer 2020-01, Vol.11 (20), p.6122-6132
Main Authors: Dai, Jiaqi, Yu, Xiaqing, Han, Yali, Chai, Li, Liao, Yina, Zhong, Peng, Xie, Ruting, Sun, Xuechen, Huang, Qingqing, Wang, Jian, Yin, Zhiqiang, Zhang, Yun, Lv, Zhongwei, Jia, Chengyou
Format: Article
Language:English
Subjects:
Antibodies
Bioinformatics
Chromatography
Datasets
Disease
Gene expression
Genomics
Labeling
Lymphatic system
Mass spectrometry
Metastasis
Mutation
Polypeptides
Proteins
Proteomics
Research Paper
Scientific imaging
Software
Thyroid cancer
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Summary:Background and Aim: Invasion and metastasis are critical events in papillary thyroid carcinoma (PTC) progression. Protein markers specific to this process may avoid over-treatment and urgently needed. Methods: TMT-labeled mass spectrometry-based proteomics were carried out on PTC and invasive phenotype (iPTC) (3 pairs per group) and cross validate differentially expressed proteins (DEPs) (FC> 1.5 and < 0.67 and p< 0.05) with GEO and TCGA datasets and the correlation genes of DEPs were also analyzed. Results: We identified and quantified 4607 proteins identical to PTC and iPTC groups. Among which 12 DEPs in PTC and 179 DEPs in iPTCs were found. Cross-validation with GSE60542 and TCGA database revealed 10 DEPs that all significant correlated with metastasis and staging. Upregulated SLC27A6 showed negative correlation with 6 out of 9 downregulated DEPs including HGD, CA4, COL23A1, SLC26A7, FHL1 and TPO. Conclusion: The panel of 7 genes (SLC27A6 and 6 downregulated DEPs) could have ideal prediction value to improve our understanding of invasiveness of PTC.
ISSN:1837-9664
1837-9664
DOI:10.7150/jca.47290