Overexpression of miR-17 is correlated with liver metastasis in colorectal cancer

Colorectal cancer (CRC) is the second leading cause of cancer-related deaths in men and women. The presence of systemic disease, with metastatic spread to distant sites such as the liver, considerably reduces the survival rate in CRC. Cancer stem cells contribute to the metastatic potential of CRC....

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Published in:Medicine (Baltimore) 2020-02, Vol.99 (9), p.e19265-e19265
Main Authors: Lai, Hao, Zhang, Jie, Zuo, Hongqun, Liu, Haizhou, Xu, Jing, Feng, Yan, Lin, Yuan, Mo, Xianwei
Format: Article
Language:English
Subjects:
Clinical Trial/Experimental Study
Colorectal Neoplasms - blood
Colorectal Neoplasms - pathology
Female
Gene Expression Regulation, Neoplastic
Humans
Liver Neoplasms - blood
Liver Neoplasms - secondary
Male
MicroRNAs - blood
MicroRNAs - metabolism
Middle Aged
Neoplasm Metastasis
Polymerase Chain Reaction
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container_title Medicine (Baltimore)
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creator Lai, Hao
Zhang, Jie
Zuo, Hongqun
Liu, Haizhou
Xu, Jing
Feng, Yan
Lin, Yuan
Mo, Xianwei
description Colorectal cancer (CRC) is the second leading cause of cancer-related deaths in men and women. The presence of systemic disease, with metastatic spread to distant sites such as the liver, considerably reduces the survival rate in CRC. Cancer stem cells contribute to the metastatic potential of CRC. However, the mechanism underlying metastasis in CRC remains unclear. Thus, this study aimed to examine the expression of microRNAs (miRNAs) in CRC stem cells in cases of liver metastases and assess their correlation with clinicopathological features. miRNAs showing high expression in liver metastases and primary lesions were selected through data mining of gene expression omnibus datasets, and miRNAs characteristic of stem cells were selected through COREMINE medical text mining. Subsequently, paired formalin-fixed paraffin-embedded tissue samples of primary CRC and liver metastasis from 30 patients were examined for the expression of miRNAs common to these lists (hsa-miR-20a, hsa-miR-26b, hsa-miR-146a, hsa-miR-17, hsa-miR-451, hsa-miR-23a, and hsa-miR-29a) using quantitative real-time polymerase chain reaction. Further, miRNA expression was compared between liver metastases and the primary tumor in each patient and the factors associated with differential expression were analyzed. hsa-miR-17 was significantly upregulated in liver metastases (P 
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The presence of systemic disease, with metastatic spread to distant sites such as the liver, considerably reduces the survival rate in CRC. Cancer stem cells contribute to the metastatic potential of CRC. However, the mechanism underlying metastasis in CRC remains unclear. Thus, this study aimed to examine the expression of microRNAs (miRNAs) in CRC stem cells in cases of liver metastases and assess their correlation with clinicopathological features. miRNAs showing high expression in liver metastases and primary lesions were selected through data mining of gene expression omnibus datasets, and miRNAs characteristic of stem cells were selected through COREMINE medical text mining. Subsequently, paired formalin-fixed paraffin-embedded tissue samples of primary CRC and liver metastasis from 30 patients were examined for the expression of miRNAs common to these lists (hsa-miR-20a, hsa-miR-26b, hsa-miR-146a, hsa-miR-17, hsa-miR-451, hsa-miR-23a, and hsa-miR-29a) using quantitative real-time polymerase chain reaction. Further, miRNA expression was compared between liver metastases and the primary tumor in each patient and the factors associated with differential expression were analyzed. hsa-miR-17 was significantly upregulated in liver metastases (P &lt; .05), but no significant difference in the expression of hsa-miR-26b, hsa-miR-146a, hsa-miR-451, hsa-miR-23a, and hsa-miR-29a was observed between primary tumors and liver metastases. The higher expression of hsa-miR-17 in liver metastases was associated with the administration of neoadjuvant chemotherapy and tumor differentiation (P &lt; .05) but was not associated with age, sex, tumor location, or lymphatic metastasis. High expression of miR-17 may contribute to liver metastasis in CRC. Therefore, an in-depth understanding of its downstream pathways could help in elucidating the mechanisms underlying liver metastases in CRC. However, additional studies are warranted to validate these findings.</description><identifier>ISSN: 0025-7974</identifier><identifier>EISSN: 1536-5964</identifier><identifier>DOI: 10.1097/MD.0000000000019265</identifier><identifier>PMID: 32118734</identifier><language>eng</language><publisher>United States: Wolters Kluwer Health</publisher><subject>Clinical Trial/Experimental Study ; Colorectal Neoplasms - blood ; Colorectal Neoplasms - pathology ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Liver Neoplasms - blood ; Liver Neoplasms - secondary ; Male ; MicroRNAs - blood ; MicroRNAs - metabolism ; Middle Aged ; Neoplasm Metastasis ; Polymerase Chain Reaction</subject><ispartof>Medicine (Baltimore), 2020-02, Vol.99 (9), p.e19265-e19265</ispartof><rights>Copyright © 2020 the Author(s). 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The presence of systemic disease, with metastatic spread to distant sites such as the liver, considerably reduces the survival rate in CRC. Cancer stem cells contribute to the metastatic potential of CRC. However, the mechanism underlying metastasis in CRC remains unclear. Thus, this study aimed to examine the expression of microRNAs (miRNAs) in CRC stem cells in cases of liver metastases and assess their correlation with clinicopathological features. miRNAs showing high expression in liver metastases and primary lesions were selected through data mining of gene expression omnibus datasets, and miRNAs characteristic of stem cells were selected through COREMINE medical text mining. Subsequently, paired formalin-fixed paraffin-embedded tissue samples of primary CRC and liver metastasis from 30 patients were examined for the expression of miRNAs common to these lists (hsa-miR-20a, hsa-miR-26b, hsa-miR-146a, hsa-miR-17, hsa-miR-451, hsa-miR-23a, and hsa-miR-29a) using quantitative real-time polymerase chain reaction. Further, miRNA expression was compared between liver metastases and the primary tumor in each patient and the factors associated with differential expression were analyzed. hsa-miR-17 was significantly upregulated in liver metastases (P &lt; .05), but no significant difference in the expression of hsa-miR-26b, hsa-miR-146a, hsa-miR-451, hsa-miR-23a, and hsa-miR-29a was observed between primary tumors and liver metastases. The higher expression of hsa-miR-17 in liver metastases was associated with the administration of neoadjuvant chemotherapy and tumor differentiation (P &lt; .05) but was not associated with age, sex, tumor location, or lymphatic metastasis. High expression of miR-17 may contribute to liver metastasis in CRC. Therefore, an in-depth understanding of its downstream pathways could help in elucidating the mechanisms underlying liver metastases in CRC. 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The presence of systemic disease, with metastatic spread to distant sites such as the liver, considerably reduces the survival rate in CRC. Cancer stem cells contribute to the metastatic potential of CRC. However, the mechanism underlying metastasis in CRC remains unclear. Thus, this study aimed to examine the expression of microRNAs (miRNAs) in CRC stem cells in cases of liver metastases and assess their correlation with clinicopathological features. miRNAs showing high expression in liver metastases and primary lesions were selected through data mining of gene expression omnibus datasets, and miRNAs characteristic of stem cells were selected through COREMINE medical text mining. 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source LWW_医学期刊; IngentaConnect Journals; PubMed Central
subjects Clinical Trial/Experimental Study
Colorectal Neoplasms - blood
Colorectal Neoplasms - pathology
Female
Gene Expression Regulation, Neoplastic
Humans
Liver Neoplasms - blood
Liver Neoplasms - secondary
Male
MicroRNAs - blood
MicroRNAs - metabolism
Middle Aged
Neoplasm Metastasis
Polymerase Chain Reaction
title Overexpression of miR-17 is correlated with liver metastasis in colorectal cancer
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