Apelin inhibits an endothelium-derived hyperpolarizing factor-like pathway in rat cerebral arteries

• Published in: Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2020-10, Vol.132, p.170350-170350, Article 170350
• Main Authors: Mughal, Amreen, Anto, Santo, Sun, Chengwen, O’Rourke, Stephen T.
• Format: Article
• Language: English
• Subjects: Apelin; BKCa channels; Cerebral artery; EDHF; EETs; Vasorelaxation
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2020.170350

•Apelin causes vasodilation in most arteries.•In cerebral arteries, apelin inhibits endothelium-dependent relaxation•This is due to impaired activation of smooth muscle BKCa channels by EETs.•Inhibition of this pathway may create an environment favoring vasoconstriction in cerebral arteries. Apelin has complex vasomotor actions inasmuch as the peptide may cause either vasodilation or vasoconstriction depending on the vascular bed and experimental conditions. In cerebral arteries, apelin inhibits endothelium-dependent relaxations mediated by nitric oxide (NO); however, its effects on relaxation to other endothelium-derived substances (e.g. prostacyclin, endothelium-derived hyperpolarizing factors(s) (EDHF)) are unknown. The present study was designed to determine effects of apelin on endothelium-dependent relaxations that are independent of NO in rat cerebral arteries. In arterial rings contracted with 5-HT, A23187 caused endothelium-dependent relaxation that was unaffected by inhibitors of eNOS, guanylyl cyclase or cyclooxygenase, but was attenuated by MS-PPOH, a selective inhibitor of cytochrome P450 catalyzed synthesis of epoxyeicosatrienoic acids (EETs) and by 14,15-EE(Z)E, an EET-receptor antagonist. Apelin inhibited A23187-induced relaxation, as well as relaxations evoked by exogenous 11,12- and 14,15-EET. These effects of apelin were mimicked by the selective BKCa channel blocker, iberiotoxin. The APJ receptor antagonist, F13A abolished the effects of apelin on A23187-induced relaxations. Both 11,12- and 14,15-EET also increased BKCa channel current density in isolated cerebral artery smooth muscle cells, effects that were inhibited in a similar manner by apelin and iberiotoxin. These findings provide evidence that apelin impairs endothelium-dependent relaxation of cerebral arteries by inhibiting an NO-independent pathway (i.e. “EDHF-like”) involving activation of smooth muscle cell BKCa channels by endothelium-derived EETs. Inhibition of such pathway may create an environment favoring vasoconstriction in cerebral arteries.