In Vivo Quasi-Elastic Light Scattering Eye Scanner Detects Molecular Aging in Humans

Abstract The absence of clinical tools to evaluate individual variation in the pace of aging represents a major impediment to understanding aging and maximizing health throughout life. The human lens is an ideal tissue for quantitative assessment of molecular aging in vivo. Long-lived proteins in le...

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Published in:The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 2020-09, Vol.75 (9), p.e53-e62
Main Authors: Minaeva, Olga, Sarangi, Srikant, Ledoux, Danielle M, Moncaster, Juliet A, Parsons, Douglas S, Washicosky, Kevin J, Black, Caitlin A, Weng, Frank J, Ericsson, Maria, Moir, Robert D, Tripodis, Yorghos, Clark, John I, Tanzi, Rudolph E, Hunter, David G, Goldstein, Lee E
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aging
Aging - physiology
Child
Child, Preschool
Cross-Sectional Studies
Crystallins - chemistry
Crystallins - physiology
Dynamic Light Scattering - methods
Electrophoresis, Polyacrylamide Gel
Eyes & eyesight
Female
Fetuses
Humans
Lens, Crystalline - physiology
Male
Microscopy, Electron, Transmission
Middle Aged
Optics
Oxidation
Oxidation-Reduction
Quasielastic light scattering
Scattering
THE JOURNAL OF GERONTOLOGY: Virtual Translational Section: Visionary Aging
Young Adult
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Summary:Abstract The absence of clinical tools to evaluate individual variation in the pace of aging represents a major impediment to understanding aging and maximizing health throughout life. The human lens is an ideal tissue for quantitative assessment of molecular aging in vivo. Long-lived proteins in lens fiber cells are expressed during fetal life, do not undergo turnover, accumulate molecular alterations throughout life, and are optically accessible in vivo. We used quasi-elastic light scattering (QLS) to measure age-dependent signals in lenses of healthy human subjects. Age-dependent QLS signal changes detected in vivo recapitulated time-dependent changes in hydrodynamic radius, protein polydispersity, and supramolecular order of human lens proteins during long-term incubation (~1 year) and in response to sustained oxidation (~2.5 months) in vitro. Our findings demonstrate that QLS analysis of human lens proteins provides a practical technique for noninvasive assessment of molecular aging in vivo.
ISSN:1079-5006
1758-535X
DOI:10.1093/gerona/glaa121