The NRF2/KEAP1 Pathway Modulates Nasopharyngeal Carcinoma Cell Radiosensitivity via ROS Elimination

Radioresistance is a vital obstacle for the prognosis of human nasopharyngeal carcinoma (NPC), but the underlying mechanism is still unknown. Here, we explored the role of the NRF2/KEAP1 pathway in radioresistance of NPC cell lines. We selected NPC cell lines CNE-1 and CNE-2, treated them with ioniz...

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Bibliographic Details
Published in:OncoTargets and therapy 2020-01, Vol.13, p.9113-9122
Main Authors: Zhou, Jieyu, Ding, Jiping, Ma, Xingkai, Zhang, Meichao, Huo, Zirong, Yao, Yuan, Li, Dong, Wang, Zhentao
Format: Article
Language:English
Subjects:
Antibodies
Antioxidants
Apoptosis
Biotechnology
Cancer
Cell growth
Cell proliferation
Cloning
Gene expression
Ionization
Nasopharyngeal carcinoma
Original Research
Radiation therapy
Radioresistance
Radiosensitivity
Reagents
Signal transduction
Throat cancer
X-rays
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Summary:Radioresistance is a vital obstacle for the prognosis of human nasopharyngeal carcinoma (NPC), but the underlying mechanism is still unknown. Here, we explored the role of the NRF2/KEAP1 pathway in radioresistance of NPC cell lines. We selected NPC cell lines CNE-1 and CNE-2, treated them with ionization, and subsequently determined the levels of NRF2, KEAP1, antioxidant enzymes, and ROS. We then evaluated the effect of NRF2 or KEAP1 inhibition on cell proliferation, colony formation, and radiosensitivity in CNE2 cells. We discovered that the NRF2/KEAP1 signaling pathway can be activated by radiotherapy in NPC cells, while knockdown enhances the sensitivity of CNE-2 cells to radiation treatment. In contrast, the silencing of inhibits the sensitivity of CNE-2 cells to radiation treatment. Our results suggest that NRF2/KEAP1 signaling may serve as an essential regulator of the radioresistance of NPC and may be applied as a novel therapeutic approach for the sensitization of NPC to radiation.
ISSN:1178-6930
1178-6930
DOI:10.2147/OTT.S260169