Hemoglobin stimulates vigorous growth of Streptococcus pneumoniae and shapes the pathogen's global transcriptome

• Published in: Scientific reports 2020-09, Vol.10 (1), p.15202, Article 15202
• Main Authors: Akhter, Fahmina, Womack, Edroyal, Vidal, Jorge E., Le Breton, Yoann, McIver, Kevin S., Pawar, Shrikant, Eichenbaum, Zehava
• Format: Article
• Language: English
• Subjects: 631/326/325/2482; 631/326/421; Bacterial Proteins - genetics; Batch Cell Culture Techniques; External stimuli; Gene Expression Profiling - methods; Gene Expression Regulation, Bacterial - drug effects; Hemoglobins - pharmacology; Humanities and Social Sciences; Iron; Isomerization; Light; Light penetration; Mathematical models; Microbial Viability - drug effects; multidisciplinary; Mutation; Orosomucoid - pharmacology; Pathogens; Science; Science (multidisciplinary); Single crystals; Streptococcus infections; Streptococcus pneumoniae; Streptococcus pneumoniae - drug effects; Streptococcus pneumoniae - genetics; Streptococcus pneumoniae - growth & development; Ultraviolet radiation
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-71910-1

Streptococcus pneumoniae (Spn) must acquire iron from the host to establish infection. We examined the impact of hemoglobin, the largest iron reservoir in the body, on pneumococcal physiology. Supplementation with hemoglobin allowed Spn to resume growth in an iron-deplete medium. Pneumococcal growth with hemoglobin was unusually robust, exhibiting a prolonged logarithmic growth, higher biomass, and extended viability in both iron-deplete and standard medium. We observed the hemoglobin-dependent response in multiple serotypes, but not with other host proteins, free iron, or heme. Remarkably, hemoglobin induced a sizable transcriptome remodeling, effecting virulence and metabolism in particular genes facilitating host glycoconjugates use. Accordingly, Spn was more adapted to grow on the human α − 1 acid glycoprotein as a sugar source with hemoglobin. A mutant in the hemoglobin/heme-binding protein Spbhp-37 was impaired for growth on heme and hemoglobin iron. The mutant exhibited reduced growth and iron content when grown in THYB and hemoglobin. In summary, the data show that hemoglobin is highly beneficial for Spn cultivation in vitro and suggest that hemoglobin might drive the pathogen adaptation in vivo. The hemoglobin receptor, Spbhp-37, plays a role in mediating the positive influence of hemoglobin. These novel findings provide intriguing insights into pneumococcal interactions with its obligate human host.