Exosomes derived from chemically induced human hepatic progenitors inhibit oxidative stress induced cell death

The emerging field of regenerative medicine has revealed that the exosome contributes to many aspects of development and disease through intercellular communication between donor and recipient cells. However, the biological functions of exosomes secreted from cells have remained largely unexplored....

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Bibliographic Details
Published in:Biotechnology and bioengineering 2020-09, Vol.117 (9), p.2658-2667
Main Authors: Hyung, Sujin, Jeong, Jaemin, Shin, Kyusoon, Kim, Ju Young, Yim, Ji‐Hye, Yu, Chan Jong, Jung, Hyun Suk, Hwang, Kyung‐Gyun, Choi, Dongho, Hong, Jong Wook
Format: Article
Language:English
Subjects:
antioxidant activity
Antioxidants
Apoptosis
Caspase
Cell death
Cell interactions
Cell signaling
cell survival
Cell viability
Cells (biology)
exosome therapy
Exosomes
Gene expression
Hepatocytes
human hepatic progenitor cells‐derived exosome (EXOhCdHs)
Hypoxia
Liver
liver disease
Mortality
Oxidative stress
Progenitor cells
Reactive oxygen species
Regeneration (physiology)
Regenerative medicine
Stem cells
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Summary:The emerging field of regenerative medicine has revealed that the exosome contributes to many aspects of development and disease through intercellular communication between donor and recipient cells. However, the biological functions of exosomes secreted from cells have remained largely unexplored. Here, we report that the human hepatic progenitor cells (CdHs)‐derived exosome (EXOhCdHs) plays a crucial role in maintaining cell viability. The inhibition of exosome secretion treatment with GW4869 results in the acceleration of reactive oxygen species (ROS) production, thereby causing a decrease of cell viability. This event provokes inhibition of caspase dependent cell death signaling, leading to a ROS‐dependent cell damage response and thus induces promotion of antioxidant gene expression or repair of cell death of hypoxia‐exposed cells. Together, these findings show the effect of exosomes in regeneration of liver cells, and offer valuable new insights into liver regeneration. Protective effect of hCdHs‐derived exosome against oxidative stress in hepatocyte. Exosomes were isolated from chemically induced human hepatic progenitors (hCdHs) by using ‘H’ method. hCdHs‐derived exosome (EXOhCdHs) has a beneficial effect on antioxidant activity of damaged hepatocytes.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.27447