DNA Gyrase and Topoisomerase IV Mutations and their effect on Quinolones Resistant Proteus mirabilis among UTIs Patients

• Published in: Pakistan journal of medical sciences 2020-10, Vol.36 (6), p.1234-1240
• Main Authors: Abdelkreem, Randa H, Yousuf, Amjad M, Elmekki, Miskelyemen A, Elhassan, Mogahid M
• Format: Article
• Language: English
• Subjects: Amino acids; Antibiotics; Care and treatment; Ciprofloxacin; Deoxyribonucleic acid; DNA; Genes; Genetic aspects; Lactose; Microbial drug resistance; Mutation; Original; Polymerase chain reaction; Urinary tract infections; Virulence
• ISSN: 1682-024X; 1681-715X
• DOI: 10.12669/pjms.36.6.2207

This study aimed to highlight the importance of mutations within genome that are related to fluoroquinolone resistance. This is a cross sectional study performed in different teaching hospitals in Khartoum State from June 2016 to May 2017. A total of (120) isolates from patients with symptoms of UTIs attending different hospitals in Khartoum State were examined. First, modified Kurby Bauer method was performed for phenotypical detection of resistant isolates. Then polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) followed by sequencing were applied for detection of mutations in , , and genes of isolates. showed 30% resistance to ciprofloxacin. All samples revealed mutation at (serine 83) of and (serine 84) of by restriction endonuclease digestion. Sequencing was performed for 12 samples. For each gene, two resistant and one susceptible strains were randomly selected. The mutations associated with ciprofloxacin resistant were as follows; (1/3) (Ser 83 to Ile) and (2/3) (Ser 81 to Ile). Also it revealed silent mutations at codons of 474 leucine (3/3), 585 valine (2/3), 612 histidine (1/3) and 639 asparagine (1/3) and 469 isoleucine (2/3), 531 aspartic (2/3) and 533 glycine (1/3). Ciprofloxacin resistance in could be monitored through detection of mutations within DNA gyrase (encoded by gyrA and gyrB) and topoisomerase IV (encoded by parC and parE).