Fitness Cost and Compensatory Evolution in Levofloxacin-Resistant Mycobacterium aurum

We isolated spontaneous levofloxacin-resistant strains of to study the fitness cost and compensatory evolution of fluoroquinolone resistance in mycobacteria. Five of six mutant strains with substantial growth defects showed restored fitness after being serially passaged for 18 growth cycles, along w...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2020-07, Vol.64 (8)
Main Authors: Pi, Rui, Liu, Qingyun, Takiff, Howard E, Gao, Qian
Format: Article
Language:English
Subjects:
Mechanisms of Resistance
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Summary:We isolated spontaneous levofloxacin-resistant strains of to study the fitness cost and compensatory evolution of fluoroquinolone resistance in mycobacteria. Five of six mutant strains with substantial growth defects showed restored fitness after being serially passaged for 18 growth cycles, along with increased cellular ATP level. Whole-genome sequencing identified putative compensatory mutations in the gene that restored the fitness of the resistant strains, presumably by altering the bacterial energy metabolism.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.00224-20