Loading…
Evaluation of the expression stability of reference genes in Apis mellifera under pyrethroid treatment
Honeybees ( Apis mellifera L.), which unquestionably play an economically important role in pollination and agricultural production, are at risk of decline. To study changes in gene expression in insects upon exposure to pesticides or other external stimuli, appropriate reference genes are required...
Saved in:
| Published in: | Scientific reports 2020-09, Vol.10 (1), p.16140, Article 16140 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c511t-fc62dca917e54eec07fdfaacea7f6265f8b331b68f0dd614fc8de64868b0d8353 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c511t-fc62dca917e54eec07fdfaacea7f6265f8b331b68f0dd614fc8de64868b0d8353 |
| container_end_page | |
| container_issue | 1 |
| container_start_page | 16140 |
| container_title | Scientific reports |
| container_volume | 10 |
| creator | Wieczorek, Przemysław Frąckowiak, Patryk Obrępalska-Stęplowska, Aleksandra |
| description | Honeybees (
Apis mellifera
L.), which unquestionably play an economically important role in pollination and agricultural production, are at risk of decline. To study changes in gene expression in insects upon exposure to pesticides or other external stimuli, appropriate reference genes are required for data normalization. Since there is no such gene that is absolutely invariable under all experimental conditions, the aim of this study was to identify the most stable targets suitable for subsequent normalization in quantitative experiments based on real-time polymerase chain reaction in honeybee research. Here, we evaluated the expression of fifteen candidate housekeeping genes from three breeding lines of honeybees treated with pyrethroids to identify the most stable genes. The tested insects were exposed to deltamethrin or lambda-cyhalothrin, and then, changes in the accumulation of selected transcripts were assessed, followed by statistical analyses. We concluded that
AmRPL32
,
AmACT
and
AmRPL13a
were the commonly recorded most stable genes in honeybees treated with the selected pyrethroids. |
| doi_str_mv | 10.1038/s41598-020-73125-w |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7527991</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2449448700</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-fc62dca917e54eec07fdfaacea7f6265f8b331b68f0dd614fc8de64868b0d8353</originalsourceid><addsrcrecordid>eNp9UctuFDEQtBCIRCE_wAFZ4jzg54x9QYqiAJEicYGz5bHbu45mPYPtSdi_x8smIVzwpa2u6qpWF0JvKflACVcfi6BSq44w0g2cMtndv0CnjAjZMc7Yy2f_E3Reyi1pTzItqH6NTjjTWnNOTlG4urPTamucE54DrlvA8GvJUMqhU6od4xTr_oBlCJAhOcAbSFBwTPhiiQXvYJpigyxek4eMl32Gus1z9LhmsHUHqb5Br4KdCpw_1DP04_PV98uv3c23L9eXFzedk5TWLrieeWc1HUAKAEeG4IO1DuwQetbLoEbO6dirQLzvqQhOeeiF6tVIvOKSn6FPR91lHXfgXbPOdjJLjjub92a20fyLpLg1m_nODJINWtMm8P5BIM8_VyjV3M5rTm1nw4TQQqiBkMZiR5bLcyntME8OlJhDPOYYj2nxmD_xmPs29O75bk8jj2E0Aj8SSoPSBvJf7__I_gY6Mp9f</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2449448700</pqid></control><display><type>article</type><title>Evaluation of the expression stability of reference genes in Apis mellifera under pyrethroid treatment</title><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature - nature.com Journals - Fully Open Access</source><creator>Wieczorek, Przemysław ; Frąckowiak, Patryk ; Obrępalska-Stęplowska, Aleksandra</creator><creatorcontrib>Wieczorek, Przemysław ; Frąckowiak, Patryk ; Obrępalska-Stęplowska, Aleksandra</creatorcontrib><description>Honeybees (
Apis mellifera
L.), which unquestionably play an economically important role in pollination and agricultural production, are at risk of decline. To study changes in gene expression in insects upon exposure to pesticides or other external stimuli, appropriate reference genes are required for data normalization. Since there is no such gene that is absolutely invariable under all experimental conditions, the aim of this study was to identify the most stable targets suitable for subsequent normalization in quantitative experiments based on real-time polymerase chain reaction in honeybee research. Here, we evaluated the expression of fifteen candidate housekeeping genes from three breeding lines of honeybees treated with pyrethroids to identify the most stable genes. The tested insects were exposed to deltamethrin or lambda-cyhalothrin, and then, changes in the accumulation of selected transcripts were assessed, followed by statistical analyses. We concluded that
AmRPL32
,
AmACT
and
AmRPL13a
were the commonly recorded most stable genes in honeybees treated with the selected pyrethroids.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-73125-w</identifier><identifier>PMID: 32999330</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/337/1645 ; 631/337/572 ; 631/601/1466 ; Agricultural production ; Animals ; Apis mellifera ; Bees ; Bees - genetics ; Cyhalothrin ; Deltamethrin ; Economic importance ; External stimuli ; Gene expression ; Gene Expression - drug effects ; Humanities and Social Sciences ; Insecticides ; Insects ; multidisciplinary ; Pesticides ; Pollination ; Polymerase chain reaction ; Pyrethrins - pharmacology ; Pyrethroids ; Real-Time Polymerase Chain Reaction - standards ; Reference Standards ; Science ; Science (multidisciplinary) ; Statistical analysis</subject><ispartof>Scientific reports, 2020-09, Vol.10 (1), p.16140, Article 16140</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-fc62dca917e54eec07fdfaacea7f6265f8b331b68f0dd614fc8de64868b0d8353</citedby><cites>FETCH-LOGICAL-c511t-fc62dca917e54eec07fdfaacea7f6265f8b331b68f0dd614fc8de64868b0d8353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2449448700/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2449448700?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32999330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wieczorek, Przemysław</creatorcontrib><creatorcontrib>Frąckowiak, Patryk</creatorcontrib><creatorcontrib>Obrępalska-Stęplowska, Aleksandra</creatorcontrib><title>Evaluation of the expression stability of reference genes in Apis mellifera under pyrethroid treatment</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Honeybees (
Apis mellifera
L.), which unquestionably play an economically important role in pollination and agricultural production, are at risk of decline. To study changes in gene expression in insects upon exposure to pesticides or other external stimuli, appropriate reference genes are required for data normalization. Since there is no such gene that is absolutely invariable under all experimental conditions, the aim of this study was to identify the most stable targets suitable for subsequent normalization in quantitative experiments based on real-time polymerase chain reaction in honeybee research. Here, we evaluated the expression of fifteen candidate housekeeping genes from three breeding lines of honeybees treated with pyrethroids to identify the most stable genes. The tested insects were exposed to deltamethrin or lambda-cyhalothrin, and then, changes in the accumulation of selected transcripts were assessed, followed by statistical analyses. We concluded that
AmRPL32
,
AmACT
and
AmRPL13a
were the commonly recorded most stable genes in honeybees treated with the selected pyrethroids.</description><subject>631/337/1645</subject><subject>631/337/572</subject><subject>631/601/1466</subject><subject>Agricultural production</subject><subject>Animals</subject><subject>Apis mellifera</subject><subject>Bees</subject><subject>Bees - genetics</subject><subject>Cyhalothrin</subject><subject>Deltamethrin</subject><subject>Economic importance</subject><subject>External stimuli</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>Humanities and Social Sciences</subject><subject>Insecticides</subject><subject>Insects</subject><subject>multidisciplinary</subject><subject>Pesticides</subject><subject>Pollination</subject><subject>Polymerase chain reaction</subject><subject>Pyrethrins - pharmacology</subject><subject>Pyrethroids</subject><subject>Real-Time Polymerase Chain Reaction - standards</subject><subject>Reference Standards</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Statistical analysis</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNp9UctuFDEQtBCIRCE_wAFZ4jzg54x9QYqiAJEicYGz5bHbu45mPYPtSdi_x8smIVzwpa2u6qpWF0JvKflACVcfi6BSq44w0g2cMtndv0CnjAjZMc7Yy2f_E3Reyi1pTzItqH6NTjjTWnNOTlG4urPTamucE54DrlvA8GvJUMqhU6od4xTr_oBlCJAhOcAbSFBwTPhiiQXvYJpigyxek4eMl32Gus1z9LhmsHUHqb5Br4KdCpw_1DP04_PV98uv3c23L9eXFzedk5TWLrieeWc1HUAKAEeG4IO1DuwQetbLoEbO6dirQLzvqQhOeeiF6tVIvOKSn6FPR91lHXfgXbPOdjJLjjub92a20fyLpLg1m_nODJINWtMm8P5BIM8_VyjV3M5rTm1nw4TQQqiBkMZiR5bLcyntME8OlJhDPOYYj2nxmD_xmPs29O75bk8jj2E0Aj8SSoPSBvJf7__I_gY6Mp9f</recordid><startdate>20200930</startdate><enddate>20200930</enddate><creator>Wieczorek, Przemysław</creator><creator>Frąckowiak, Patryk</creator><creator>Obrępalska-Stęplowska, Aleksandra</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20200930</creationdate><title>Evaluation of the expression stability of reference genes in Apis mellifera under pyrethroid treatment</title><author>Wieczorek, Przemysław ; Frąckowiak, Patryk ; Obrępalska-Stęplowska, Aleksandra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-fc62dca917e54eec07fdfaacea7f6265f8b331b68f0dd614fc8de64868b0d8353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>631/337/1645</topic><topic>631/337/572</topic><topic>631/601/1466</topic><topic>Agricultural production</topic><topic>Animals</topic><topic>Apis mellifera</topic><topic>Bees</topic><topic>Bees - genetics</topic><topic>Cyhalothrin</topic><topic>Deltamethrin</topic><topic>Economic importance</topic><topic>External stimuli</topic><topic>Gene expression</topic><topic>Gene Expression - drug effects</topic><topic>Humanities and Social Sciences</topic><topic>Insecticides</topic><topic>Insects</topic><topic>multidisciplinary</topic><topic>Pesticides</topic><topic>Pollination</topic><topic>Polymerase chain reaction</topic><topic>Pyrethrins - pharmacology</topic><topic>Pyrethroids</topic><topic>Real-Time Polymerase Chain Reaction - standards</topic><topic>Reference Standards</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Statistical analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wieczorek, Przemysław</creatorcontrib><creatorcontrib>Frąckowiak, Patryk</creatorcontrib><creatorcontrib>Obrępalska-Stęplowska, Aleksandra</creatorcontrib><collection>SpringerOpen</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wieczorek, Przemysław</au><au>Frąckowiak, Patryk</au><au>Obrępalska-Stęplowska, Aleksandra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the expression stability of reference genes in Apis mellifera under pyrethroid treatment</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-09-30</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>16140</spage><pages>16140-</pages><artnum>16140</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Honeybees (
Apis mellifera
L.), which unquestionably play an economically important role in pollination and agricultural production, are at risk of decline. To study changes in gene expression in insects upon exposure to pesticides or other external stimuli, appropriate reference genes are required for data normalization. Since there is no such gene that is absolutely invariable under all experimental conditions, the aim of this study was to identify the most stable targets suitable for subsequent normalization in quantitative experiments based on real-time polymerase chain reaction in honeybee research. Here, we evaluated the expression of fifteen candidate housekeeping genes from three breeding lines of honeybees treated with pyrethroids to identify the most stable genes. The tested insects were exposed to deltamethrin or lambda-cyhalothrin, and then, changes in the accumulation of selected transcripts were assessed, followed by statistical analyses. We concluded that
AmRPL32
,
AmACT
and
AmRPL13a
were the commonly recorded most stable genes in honeybees treated with the selected pyrethroids.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32999330</pmid><doi>10.1038/s41598-020-73125-w</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2020-09, Vol.10 (1), p.16140, Article 16140 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7527991 |
| source | Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central; Free Full-Text Journals in Chemistry; Springer Nature - nature.com Journals - Fully Open Access |
| subjects | 631/337/1645 631/337/572 631/601/1466 Agricultural production Animals Apis mellifera Bees Bees - genetics Cyhalothrin Deltamethrin Economic importance External stimuli Gene expression Gene Expression - drug effects Humanities and Social Sciences Insecticides Insects multidisciplinary Pesticides Pollination Polymerase chain reaction Pyrethrins - pharmacology Pyrethroids Real-Time Polymerase Chain Reaction - standards Reference Standards Science Science (multidisciplinary) Statistical analysis |
| title | Evaluation of the expression stability of reference genes in Apis mellifera under pyrethroid treatment |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T16%3A14%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20the%20expression%20stability%20of%20reference%20genes%20in%20Apis%20mellifera%20under%20pyrethroid%20treatment&rft.jtitle=Scientific%20reports&rft.au=Wieczorek,%20Przemys%C5%82aw&rft.date=2020-09-30&rft.volume=10&rft.issue=1&rft.spage=16140&rft.pages=16140-&rft.artnum=16140&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-020-73125-w&rft_dat=%3Cproquest_pubme%3E2449448700%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c511t-fc62dca917e54eec07fdfaacea7f6265f8b331b68f0dd614fc8de64868b0d8353%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2449448700&rft_id=info:pmid/32999330&rfr_iscdi=true |