Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats

A rat model of secondary osteoporosis was constructed using retinoic acid as an inducer, and the genes, proteins, and bone mass of the rats were analyzed. qPCR detection of the Wnt/β-catenin and OPG/RANK/RANKL signaling pathway-related gene expression levels showed that Lactobacillus plantarum HFY15...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2020, Vol.2020 (2020), p.1-10
Main Authors: Zhao, Xin, Mu, Jianfei, Yi, Ruokun, Li, Fang, Zheng, Jiazhuang, Liu, Xinhong, Tan, Fang
Format: Article
Language:English
Subjects:
Acid phosphatase (tartrate-resistant)
Animal models
Bone density
Bone mass
Bone mineral density
Cancellous bone
Cbfa-1 protein
Computed tomography
Cytokines
Deoxyribonucleic acid
Dkk1 protein
DNA
Drug dosages
Enzymes
Fractures
Gene expression
Laboratory animals
LRP5 protein
Morphology
Osteoporosis
Osteoprotegerin
Phosphatase
Phosphatases
Probiotics
Retinoic acid
Rodents
Signal transduction
Thyroid gland
TRANCE protein
Tretinoin
Wnt protein
Yogurt
Zoledronic acid
β-Catenin
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Summary:A rat model of secondary osteoporosis was constructed using retinoic acid as an inducer, and the genes, proteins, and bone mass of the rats were analyzed. qPCR detection of the Wnt/β-catenin and OPG/RANK/RANKL signaling pathway-related gene expression levels showed that Lactobacillus plantarum HFY15 played a positive role in regulating both pathways. HFY15 significantly increased β-catenin, Lrp5, Lrp6, Wnt10b, OPG, RANKL, and Runx2 expression and downregulated DKK1, RANK, CTSK, TRACP, and ALP expression. Enzyme-linked immunosorbent assays further confirmed the qPCR results. Tartrate-resistant acid phosphatase staining showed that HFY15 slowed retinoic acid-induced osteoclast formation. Microcomputed tomography showed that HFY15 reduced trabecular separation and increased the percent bone volume, trabecular numbers, trabecular thickness, and bone mineral density in the rats in vivo. These findings indicate that HFY15 may help prevent retinoic acid-induced secondary osteoporosis in vivo.
ISSN:1741-427X
1741-4288
DOI:10.1155/2020/2054389