COL3A1, COL6A3, and SERPINH1 Are Related to Glucocorticoid-Induced Osteoporosis Occurrence According to Integrated Bioinformatics Analysis

BACKGROUND Glucocorticoid-induced osteoporosis (GIOP) represents the most frequently seen type of secondary osteoporosis, a systemic skeleton disorder. Numerous factors are associated with GIOP occurrence, but there are no specific diagnostic and therapeutic biomarkers for GIOP so far. MATERIAL AND...

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Bibliographic Details
Published in:Medical science monitor 2020-10, Vol.26, p.e925474-e925474
Main Authors: Li, Liuxun, Yang, Meiling, Jin, Anmin
Format: Article
Language:English
Subjects:
Biomarkers - metabolism
Collagen Type III - biosynthesis
Collagen Type III - genetics
Collagen Type VI - biosynthesis
Collagen Type VI - genetics
Computational Biology
Database Analysis
Databases, Nucleic Acid
Female
Glucocorticoids - administration & dosage
Glucocorticoids - adverse effects
HSP47 Heat-Shock Proteins - biosynthesis
HSP47 Heat-Shock Proteins - genetics
Humans
Male
Osteoporosis - chemically induced
Osteoporosis - genetics
Osteoporosis - metabolism
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Summary:BACKGROUND Glucocorticoid-induced osteoporosis (GIOP) represents the most frequently seen type of secondary osteoporosis, a systemic skeleton disorder. Numerous factors are associated with GIOP occurrence, but there are no specific diagnostic and therapeutic biomarkers for GIOP so far. MATERIAL AND METHODS In this work, gene modules related to GIOP were screened through weighted gene coexpression network analysis. Moreover, protein-protein interaction (PPI) networks and gene set enrichment analysis (GSEA) were carried out for hub genes. In addition, microarray GSE30159 dataset was used as a training set to analyze gene expression within bone biopsy samples from patients with endogenous Cushing's syndrome with GIOP and from normal controls. GSE129228 was used as the test set for investigating the hub gene involvement within GIOP. RESULTS According to our results, the turquoise module showed clinical significance, and 10 genes (COL3A1, POSTN, COL6A3, COL14A1, SERPINH1, ASPN, OGN, THY1, NID2, and TNMD) were discovered to be the "real" hub genes within coexpression as well as PPI networks. GSEA showed that the interaction of extracellular matrix receptors together with the focal adhesion pathway had significant enrichment within samples with high COL3A1 and COL6A3 expression. After the results from both test and training sets were overlapped, SERPINH1 was also significantly altered between GIOP and normal control samples. CONCLUSIONS COL3A1, COL6A3, and SERPINH1 were identified to be the candidate biomarkers for GIOP.
ISSN:1643-3750
1234-1010
1643-3750
DOI:10.12659/MSM.925474