Overproduction of a Dominant Mutant of the Conserved Era GTPase Inhibits Cell Division in Escherichia coli

• Published in: Journal of bacteriology 2020-10, Vol.202 (21)
• Main Authors: Zhou, Xiaomei, Peters, 3rd, Howard K, Li, Xintian, Costantino, Nina, Kumari, Vandana, Shi, Genbin, Tu, Chao, Cameron, Todd A, Haeusser, Daniel P, Vega, Daniel E, Ji, Xinhua, Margolin, William, Court, Donald L
• Format: Article
• Language: English
• Subjects: Alleles; Bacterial Proteins - metabolism; Bacteriology; Cell Cycle Checkpoints; Cell Cycle Proteins - metabolism; Cell Division; Circuits; Cytoskeletal Proteins - metabolism; E coli; Escherichia coli; Escherichia coli - cytology; Escherichia coli - metabolism; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Filamentation; Growth rate; GTP-Binding Proteins - genetics; GTP-Binding Proteins - metabolism; Homeostasis; Mutant Proteins - metabolism; Mutants; Phenotypes; Proteins; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Suppressors
• ISSN: 0021-9193; 1098-5530
• DOI: 10.1128/JB.00342-20

Cell growth and division are coordinated, ensuring homeostasis under any given growth condition, with division occurring as cell mass doubles. The signals and controlling circuit(s) between growth and division are not well understood; however, it is known in that the essential GTPase Era, which is growth rate regulated, coordinates the two functions and may be a checkpoint regulator of both. We have isolated a mutant of Era that separates its effect on growth and division. When overproduced, the mutant protein Era647 is dominant to wild-type Era and blocks division, causing cells to filament. Multicopy suppressors that prevent the filamentation phenotype of Era647 either increase the expression of FtsZ or decrease the expression of the Era647 protein. Excess Era647 induces complete delocalization of Z rings, providing an explanation for why Era647 induces filamentation, but this effect is probably not due to direct interaction between Era647 and FtsZ. The hypermorphic * allele at the native locus can suppress the effects of Era647 overproduction, indicating that extra FtsZ is not required for the suppression, but another hypermorphic allele that accelerates cell division through periplasmic signaling, *, cannot. Together, these results suggest that Era647 blocks cell division by destabilizing the Z ring. All cells need to coordinate their growth and division, and small GTPases that are conserved throughout life play a key role in this regulation. One of these, Era, provides an essential function in the assembly of the 30S ribosomal subunit in , but its role in regulating cell division is much less well understood. Here, we characterize a novel dominant negative mutant of Era (Era647) that uncouples these two activities when overproduced; it inhibits cell division by disrupting assembly of the Z ring, without significantly affecting ribosome production. The unique properties of this mutant should help to elucidate how Era regulates cell division and coordinates this process with ribosome biogenesis.