Effects of Launaea sarmentosa Extract on Lipopolysaccharide-Induced Inflammation via Suppression of NF-κB/MAPK Signaling and Nrf2 Activation

• Published in: Nutrients 2020-08, Vol.12 (9), p.2586
• Main Authors: Nguyen, Thanh Q C, Binh, Tran Duy, Kusunoki, Ryo, Pham, Tuan L A, Nguyen, Yen D H, Nguyen, Trong Tuan, Kanaori, Kenji, Kamei, Kaeko
• Format: Article
• Language: English
• Subjects: Activation; AKT protein; Animals; Anti-Inflammatory Agents - pharmacology; Antioxidants; Cell culture; Cytokines; Cytotoxicity; Dehydrogenases; Endoplasmic reticulum; Enzymes; Flavonoids; Heme; Heme oxygenase (decyclizing); Herbal medicine; Inflammation; Inflammation - drug therapy; Inflammatory diseases; Inflammatory response; Kinases; Launaea; Lepidopora sarmentosa; Lipopolysaccharides; Macrophages; MAP kinase; MAP Kinase Signaling System - drug effects; Medical treatment; Medicine, Traditional; Mice; Molecular modelling; NF-E2-Related Factor 2 - drug effects; NF-kappa B - drug effects; NF-κB protein; Nitric oxide; Oxidative stress; Oxygenase; Phenols; Phosphorylation; Plant Extracts - pharmacology; Pretreatment; Proteins; RAW 264.7 Cells; Reactive oxygen species; Reagents; Signal Transduction - drug effects; Tumor necrosis factor-TNF; Tumor necrosis factor-α
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu12092586

has been extensively used as a nutrient herb in traditional Vietnamese remedies for the treatment of various diseases, especially inflammatory diseases. However, no detailed research has been conducted examining the molecular mechanisms involved in the suppression of inflammatory response. Here, we studied the effects of methanol extract on lipopolysaccharide (LPS)-induced inflammation using RAW 264.7 macrophages. The extract demonstrated potent antioxidant activity owing to the presence of polyphenolic and flavonoid components. Pretreatment with the extract inhibited LPS-mediated secretion of nitric oxide, reactive oxygen species, and tumor necrosis factor-α as well as the expression of inflammatory cytokines. Furthermore, the activation of the nuclear factor-kappa B pathway and phosphoinositide-3-kinase/protein kinase B pathways was blocked by the extract by inhibiting Akt phosphorylation. Additionally, the mitogen-activated protein kinase pathway was suppressed, and endoplasmic reticulum stress was attenuated. Furthermore, the extract promoted the activity of nuclear factor erythroid-2-related factor 2 resulting in the up-regulation of heme oxygenase-1 pathway, leading to the suppression of oxidative stress and inflammatory response. Taken together, the results indicate that exhibits anti-inflammatory effects, and hence, can be further developed as a novel drug for the treatment of diseases associated with excessive inflammation.