Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols

This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individ...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2021-04, Vol.40 (4), p.889-892
Main Authors: Volpato, Fabiana, Lima-Morales, Daiana, Wink, Priscila Lamb, Willig, Julia, de-Paris, Fernanda, Ashton-Prolla, Patricia, Barth, Afonso Luís
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Brief Report
Comparative analysis
COVID-19
COVID-19 - diagnosis
COVID-19 Nucleic Acid Testing - methods
Diagnostic systems
Humans
Internal Medicine
Medical Microbiology
Polymerase chain reaction
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Specimen Handling - methods
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Summary:This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individual results. However, for results from protocol B, reduced agreement (91%) was observed in relation to individual testing. Inconsistencies observed were related to RT-qPCR results with higher cycle thresholds. These results suggest that pooling of samples before RNA extraction is preferable in terms of diagnostic for SARS-CoV-2.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-020-04071-8