Evaluation of PD-L1 Expression and HPV Genotyping in Anal Squamous Cell Carcinoma

Anal squamous cell carcinoma (SCC) is a rare cancer with increasing incidence. Infection with high-risk human papillomavirus (HPV) subtypes is the major cause for its development. We retrospectively analyzed tumor samples from 54 anal SCC patients for infection with a panel of 32 HPV subtypes in a P...

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Bibliographic Details
Published in:Cancers 2020-09, Vol.12 (9), p.2516
Main Authors: Wessely, Anja, Heppt, Markus V., Kammerbauer, Claudia, Steeb, Theresa, Kirchner, Thomas, Flaig, Michael J., French, Lars E., Berking, Carola, Schmoeckel, Elisa, Reinholz, Markus
Format: Article
Language:English
Subjects:
Anus
Biomarkers
Cancer therapies
Cell cycle
Colorectal cancer
Gene expression
Genetic aspects
Genotypes
Genotyping
Health aspects
HIV
Human immunodeficiency virus
Human papillomavirus
Infections
Melanoma
Papillomavirus infections
Patients
PD-L1 protein
Squamous cell carcinoma
Survival analysis
Tumors
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Summary:Anal squamous cell carcinoma (SCC) is a rare cancer with increasing incidence. Infection with high-risk human papillomavirus (HPV) subtypes is the major cause for its development. We retrospectively analyzed tumor samples from 54 anal SCC patients for infection with a panel of 32 HPV subtypes in a PCR-based approach, determined the PD-L1 expression status, and correlated the findings with the clinical data and the survival of the patients. Forty-two patients (77.8%) were HPV-positive and harbored at least one carcinogenic HPV subtype. HPV16 was the most frequently detected (n = 39, 72.2%). Four patients were infected with multiple HPV subtypes. HPV infection was significantly more often detected in female than in male patients (90.3% vs. 60.9%, p = 0.018). Patients with PD-L1 positive tumors showed a significantly better median overall survival (OS) compared with patients with PD-L1 negative tumors (69.3 vs. 28.3 months, p = 0.006). The median OS was significantly different among the distinct tumor stages (p = 0.029). Sex, grade of differentiation, and HPV infection status did not influence the median OS. Furthermore, HPV infection status and PD-L1 expression were not correlated. A multivariate Cox regression analysis revealed that PD-L1 expression status was an independent prognostic marker for survival (p = 0.012).
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers12092516