Production of Chitosan/Hyaluronan Complex Nanofibers. Characterization and Physical Properties as a Function of the Composition

In this work, optimized conditions for preparation of chitosan and hyaluronan polyelectrolyte complex are proposed. The objective was to produce new biomaterials being biocompatible and bioresorbable in the body as well as approaching the extracellular matrix (ECM) structure. These materials will be...

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Bibliographic Details
Published in:Polymers 2020-09, Vol.12 (9), p.2004
Main Authors: Garcia Garcia, Christian Enrique, Soltero Martínez, Félix Armando, Bossard, Frédéric, Rinaudo, Marguerite
Format: Article
Language:English
Subjects:
Biocompatibility
Biomedical materials
Cartilage
Chitosan
Composition
Crosslinking
Engineering Sciences
Heat treatment
Hyaluronic acid
Mechanical properties
Molecular weight
Nanofibers
Physical properties
Polyelectrolytes
Polyethylene
Polymers
Polysaccharides
Solvents
Swelling
Tissue engineering
Wound healing
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Summary:In this work, optimized conditions for preparation of chitosan and hyaluronan polyelectrolyte complex are proposed. The objective was to produce new biomaterials being biocompatible and bioresorbable in the body as well as approaching the extracellular matrix (ECM) structure. These materials will be tested for chondrocyte development in tissue engineering and wound healing applications. Nanofibers made of the polyelectrolyte complex (PEC) were successfully manufactured by electrospinning, and casted films were used as a model for properties comparison. To our knowledge, it is the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4. The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation. The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances. The influence of the solvent (acidic potential and composition) utilized to process biomaterials is also examined. Acid formic/water 50/50 is observed to be the more appropriated solvent for the carried-out procedures.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym12092004